Lantos T A, Görcs T J, Palkovits M
Laboratory of Neuromorphology, Semmelweis University Medical School, Budapest, Hungary.
Brain Res Brain Res Rev. 1995 Feb;20(2):209-49. doi: 10.1016/0165-0173(94)00013-f.
The topographical distribution of neuropeptide-containing cell bodies, fibers and terminals was studied in the premamillary region of the rat hypothalamus using light microscopic immunohistochemistry. Alternate coronal sections through the posterior third of the hypothalamus of normal and colchicine-treated male rats were immunostained for 19 different neuropeptides and their distributions were mapped throughout the following structures: the ventral and dorsal premamillary, the supramamillary, the tuberomamillary and the posterior hypothalamic nuclei, as well as the premamillary portion of the arcuate nucleus and the postinfundibular median eminence. Seventeen of the investigated neuropeptides were present in neuronal perikarya, nerve fibers and terminals while the gonadotropin associated peptide and vasopressin occurred only in fibers and terminals. Growth hormone-releasing hormone-, somatostatin-, alpha-melanocyte stimulating hormone-, adrenocorticotropin-, beta-endorphin- and neuropeptide Y-immunoreactive neurons were seen exclusively in the premamillary portion of the arcuate nucleus. Thyrotropin-releasing hormone-, dynorphin A- and galanin-containing neurons were distributed mainly in the arcuate and the tuberomamillary nuclei. A high number of methionine- and leucine-enkephalin-immunoreactive cells were detected in the arcuate and dorsal premamillary nuclei, as well as in the area ventrolateral to the fornix. Substance P-immunoreactive perikarya were present in very high number within the entire region, in particular in the ventral and dorsal premamillary nuclei. Cell bodies labelled with cholecystokinin- and calcitonin gene-related peptide antisera were found predominantly in the supramamillary and the terete nuclei, respectively. Corticotropin-releasing hormone-, vasoactive intestinal polypeptide- and neurotensin-immunoreactive neurons were scattered randomly in low number, mostly in the arcuate and the ventral and dorsal premamillary nuclei. Peptidergic fibers were distributed unevenly throughout the whole region, with each peptide showing an individual distribution pattern. The highest density of immunoreactive fibers was presented in the ventral half of the region including the arcuate, the ventral premamillary and the tuberomamillary nuclei. The supramamillary nucleus showed moderately dense fiber networks, while the dorsal premamillary and the posterior hypothalamic nuclei were poor in peptidergic fibers.
利用光镜免疫组织化学方法，研究了大鼠下丘脑乳头前区含神经肽的细胞体、纤维和终末的拓扑分布。对正常和秋水仙碱处理的雄性大鼠下丘脑后三分之一处的交替冠状切片进行免疫染色，检测19种不同神经肽，绘制其在以下结构中的分布图谱：腹侧和背侧乳头前核、乳头体上核、结节乳头体核和下丘脑后核，以及弓状核的乳头前部分和漏斗后正中隆起。所研究的17种神经肽存在于神经元胞体、神经纤维和终末中，而促性腺激素相关肽和血管加压素仅存在于纤维和终末中。生长激素释放激素、生长抑素、α-黑素细胞刺激激素、促肾上腺皮质激素、β-内啡肽和神经肽Y免疫反应性神经元仅见于弓状核的乳头前部分。促甲状腺激素释放激素、强啡肽A和甘丙肽免疫反应性神经元主要分布在弓状核和结节乳头体核。在弓状核、背侧乳头前核以及穹窿腹外侧区域检测到大量甲硫氨酸脑啡肽和亮氨酸脑啡肽免疫反应性细胞。P物质免疫反应性胞体在整个区域数量非常多，尤其是在腹侧和背侧乳头前核。用胆囊收缩素和降钙素基因相关肽抗血清标记的细胞体分别主要见于乳头体上核和圆柱核。促肾上腺皮质激素释放激素、血管活性肠肽和神经降压素免疫反应性神经元随机散在分布，数量较少，主要见于弓状核以及腹侧和背侧乳头前核。肽能纤维在整个区域分布不均匀，每种肽都呈现出独特的分布模式。免疫反应性纤维密度最高的区域是包括弓状核、腹侧乳头前核和结节乳头体核在内的区域下半部分。乳头体上核显示中等密度的纤维网络，而背侧乳头前核和下丘脑后核的肽能纤维较少。
