采用填充柱超临界流体色谱法对卡马西泮及其代谢物进行非手性和手性分析。

Achiral and chiral analysis of camazepam and metabolites by packed-column supercritical fluid chromatography.

作者信息

Wang M Z, Klee M S, Yang S K

机构信息

Hewlett-Packard Company, Wilmington, DE 19808, USA.

出版信息

J Chromatogr B Biomed Appl. 1995 Mar 10;665(1):139-46. doi: 10.1016/0378-4347(94)00502-v.

DOI:10.1016/0378-4347(94)00502-v

PMID:7795784

Abstract

Supercritical fluid chromatography, using carbon dioxide as the mobile phase and ethanol as a modifier, has been applied to the analysis of products formed in rat liver microsomal metabolism of racemic camazepam, a hypnotic/anxiolytic drug in clinical use. An achiral (amino) column and a chiral (Chiralcel OD-H) column were used. The results suggest that achiral and chiral packed-column supercritical fluid chromatography gives a shorter analysis time and higher selectivity and efficiency than achiral and chiral stationary-phase high-performance liquid chromatography in the analysis of camazepam and its derivatives.

摘要

超临界流体色谱法以二氧化碳为流动相、乙醇为改性剂，已应用于临床使用的催眠/抗焦虑药物消旋卡马西泮在大鼠肝微粒体代谢中形成产物的分析。使用了一根非手性（氨基）柱和一根手性（Chiralcel OD-H）柱。结果表明，在卡马西泮及其衍生物的分析中，非手性和手性填充柱超临界流体色谱法比非手性和手性固定相高效液相色谱法具有更短的分析时间、更高的选择性和效率。