用于测定猴血浆中多巴胺受体激动剂CI-1007及其代谢物PD 147693的灵敏高效液相色谱法。

Sensitive high-performance liquid chromatographic method for a dopamine receptor agonist, CI-1007, and its metabolite PD 147693 in monkey plasma.

作者信息

Feng M R, Siersma P R, Strenkoski C A, Wright D S

机构信息

Parke-Davis Pharmaceutical Research, Division of Warner-Lambert Company, Ann Arbor, MI 48105, USA.

出版信息

J Chromatogr B Biomed Appl. 1995 Mar 10;665(1):193-9. doi: 10.1016/0378-4347(94)00506-z.

DOI:10.1016/0378-4347(94)00506-z

PMID:7795791

Abstract

A sensitive gradient high-performance liquid chromatographic (HPLC) method for the simultaneous quantitation of a dopamine autoreceptor agonist CI-1007 (I) and its metabolite PD 147693 (II) is described. Monkey plasma samples were purified by liquid-liquid extraction using hexane. Liquid chromatographic separation was achieved on two C18 analytical columns (installed in series) using gradient elution. Column effluent was monitored using a fluorescence detector programmed to change wavelengths at specified times. Minimum quantitation limits of I and II were 3.0 and 5.0 ng/ml, respectively, for a plasma sample volume of 0.100 ml. Linearity was demonstrated up to 300 ng/ml. The assay has been applied to the analysis of I and II in plasma from monkeys following intravenous and oral doses of I.

摘要

描述了一种灵敏的梯度高效液相色谱（HPLC）方法，用于同时定量多巴胺自身受体激动剂CI-1007（I）及其代谢产物PD 147693（II）。猴血浆样品通过用己烷进行液-液萃取来纯化。使用梯度洗脱在两根串联的C18分析柱上实现液相色谱分离。使用荧光检测器监测柱流出物，该荧光检测器被编程为在指定时间改变波长。对于0.100 ml的血浆样品体积，I和II的最低定量限分别为3.0和5.0 ng/ml。线性范围证明可达300 ng/ml。该测定法已应用于静脉内和口服给予I后猴血浆中I和II的分析。