Garibotti M, Christiansen H, Schmale H, Pelosi P
Istituto di Industrie Agrarie, Pisa, Italy.
Chem Senses. 1995 Feb;20(1):69-76. doi: 10.1093/chemse/20.1.69.
Small soluble proteins, belonging to the lipocalin family are secreted in large amounts by tongue von Ebner's glands and lachrymal glands. In humans, the lingual protein, called VEG, and the lachrymal protein, called tear prealbumin, have shown identical cDNA sequences. In the pig, we have purified homodimeric proteins with subunits of 17 kDa, both from von Ebner's glands and from lachrymal glands. In both cases, the proteins can be resolved into two isoforms on a chromatofocusing column. Partial aminoacid sequences and full cDNA sequences have been obtained for the more abundant forms purified from both tissues. The two proteins appear to be identical, as in humans. The reason why the same protein is expressed in different tissues, as well as its physiological function, still remain to be clarified.
属于脂质运载蛋白家族的小可溶性蛋白质由舌部的埃伯纳氏腺和泪腺大量分泌。在人类中，舌部蛋白质称为VEG，泪腺蛋白质称为泪前白蛋白，它们具有相同的cDNA序列。在猪中，我们从埃伯纳氏腺和泪腺中都纯化出了亚基为17 kDa的同二聚体蛋白质。在这两种情况下，这些蛋白质在色谱聚焦柱上都可以分离成两种异构体。已经获得了从这两种组织中纯化出的含量较高的形式的部分氨基酸序列和完整的cDNA序列。与人类一样，这两种蛋白质似乎是相同的。同一蛋白质在不同组织中表达的原因及其生理功能仍有待阐明。
