Glasgow B J
Department of Pathology and Ophthalmology, Jules Stein Eye Institute, UCLA School of Medicine, Los Angeles, CA 90024, USA.
Graefes Arch Clin Exp Ophthalmol. 1995 Aug;233(8):513-22. doi: 10.1007/BF00183433.
Tear-specific prealbumin is a group of proteins recently renamed as the tear lipocalins. These proteins were initially described as unique to lacrimal fluid. The tissue distribution and localization have never been thoroughly studied.
The distribution of purified tear lipocalins was studied in many human secretions and tissues by western blots, immunohistochemistry and immunoelectron microscopy.
Tear lipocalin species of the same molecular weights were observed in western blot lanes loaded with tears, saliva, and protein extracts from the lacrimal and lingual von Ebner's glands. Lacrimal and von Ebner's glands contained tear lipocalins; other human tissues and secretions, including other salivary glands and taste buds, did not. Tear lipocalins colocalized with lysozyme in serous acinar cells of lacrimal and von Ebner's glands. Ultrastructurally, tear lipocalins were present on polyribosomes, endoplasmic reticulum, and Golgi areas. Lipocalins were concentrated in lacrimal secretory granules in amounts commensurate with a regulated pathway.
Tear lipocalins are expressed and truncated similarly in lingual von Ebner's and lacrimal glands, but not at all in other human tissues. Lipocalins are expressed and secreted with lysozyme. Lipocalins are concentrated in secretory granules in an amount consistent with a regulated secretory pathway.
泪液特异性前清蛋白是一组最近重新命名为泪液脂质运载蛋白的蛋白质。这些蛋白质最初被描述为泪液所特有的。其组织分布和定位从未得到过深入研究。
通过蛋白质免疫印迹法、免疫组织化学和免疫电子显微镜技术研究纯化的泪液脂质运载蛋白在多种人体分泌物和组织中的分布情况。
在装载有泪液、唾液以及泪腺和舌味腺蛋白质提取物的蛋白质免疫印迹条带中观察到了分子量相同的泪液脂质运载蛋白种类。泪腺和味腺含有泪液脂质运载蛋白；其他人体组织和分泌物，包括其他唾液腺和味蕾，均未含有。泪液脂质运载蛋白与溶菌酶在泪腺和味腺的浆液性腺泡细胞中共定位。在超微结构上，泪液脂质运载蛋白存在于多核糖体、内质网和高尔基体区域。脂质运载蛋白以与调节性途径相符的量浓缩在泪液分泌颗粒中。
泪液脂质运载蛋白在舌味腺和泪腺中的表达和截短情况相似，但在其他人体组织中则完全不表达。脂质运载蛋白与溶菌酶一起表达和分泌。脂质运载蛋白以与调节性分泌途径相符的量浓缩在分泌颗粒中。
