Shen K, Lang J, Guo L
Peking Union Medical College Hospital, Beijing.
Zhonghua Fu Chan Ke Za Zhi. 1995 Mar;30(3):153-6.
To determine the prevalence of p53 abnormal protein expression in transitional cell carcinoma of the ovary in Peking Union Medical College hospital, and to analyse the correlation between the overexpression of p53 protein and prognostic parameters of transitional cell carcinoma of the ovary, thirty-two transitional cell carcinoma of the ovary and 20 normal ovaries were analysed. Detection of overexpression of p53 protein was carried out by immunohistochemical staining (IHS) using the monoclonal antibody DO-1 on paraffin sections. 41% of 32 samples of transitional cell carcinoma of the ovary showed positive IHS with DO-1, whereas only 5% of 20 normal ovaries showed weakly positive cytoplasmic staining (P < 0.01). p53 overexpression did not correlate with age, CA125 levels, histological grade and lymph node metastasis, but patients with overexpression of p53 had a poor prognosis. Overexpression of p53 is a more common event in transitional cell carcinoma of the ovary. Further studies are warranted to clarify the role of p53 in transitional cell carcinoma of the ovary.
为了确定北京协和医院卵巢移行细胞癌中p53异常蛋白表达的患病率，并分析p53蛋白过表达与卵巢移行细胞癌预后参数之间的相关性，对32例卵巢移行细胞癌和20例正常卵巢进行了分析。采用单克隆抗体DO-1对石蜡切片进行免疫组织化学染色（IHS），检测p53蛋白的过表达情况。32例卵巢移行细胞癌样本中41%经DO-1免疫组织化学染色呈阳性，而20例正常卵巢中只有5%呈弱阳性细胞质染色（P<0.01）。p53过表达与年龄、CA125水平、组织学分级和淋巴结转移无关，但p53过表达的患者预后较差。p53过表达在卵巢移行细胞癌中是更常见的事件。有必要进一步研究以阐明p53在卵巢移行细胞癌中的作用。
