Arad G, Nussinovich R, Kaempfer R
Department of Molecular Virology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Immunol Lett. 1995 Jan;44(2-3):213-6. doi: 10.1016/0165-2478(94)00217-f.
Establishment of protective immunity depends critically on IFN-gamma. We show that in human peripheral blood mononuclear cells, low doses of IL-2 greatly potentiate the response of the IFN-gamma gene to mitogen, by over 100-fold. By itself, IL-2 is unable to induce IFN-gamma mRNA to a significant extent. Yet, exposure to IL-2 leads to cellular commitment within a few hours, expressed by greatly enhanced accumulation of IFN-gamma mRNA upon subsequent exposure to phytohemagglutinin. Changes induced by IL-2 do not relieve the requirement for de novo protein synthesis during the early phases of induction of IFN-gamma gene expression. IL-2 may induce a component essential for induction of IFN-gamma mRNA that is utilized during subsequent exposure to a mitogenic signal. Our results demonstrate synergy between IL-2 and mitogen in IFN-gamma gene induction.
保护性免疫的建立关键取决于干扰素-γ。我们发现，在人外周血单核细胞中，低剂量的白细胞介素-2能极大地增强干扰素-γ基因对有丝分裂原的反应，增幅超过100倍。白细胞介素-2本身在很大程度上无法诱导干扰素-γ信使核糖核酸。然而，暴露于白细胞介素-2会在数小时内导致细胞致敏，这表现为随后暴露于植物血凝素后干扰素-γ信使核糖核酸的积累大幅增加。白细胞介素-2所诱导的变化并不能在干扰素-γ基因表达诱导的早期阶段免除对从头合成蛋白质的需求。白细胞介素-2可能诱导了一种对干扰素-γ信使核糖核酸诱导至关重要的成分，该成分在随后暴露于促有丝分裂信号期间被利用。我们的结果证明了白细胞介素-2与有丝分裂原在干扰素-γ基因诱导中的协同作用。
