Ketzinel M, Efrat S, Sayar D, Gerez L, Tal C, Deutsch E, Arad G, Kaempfer R
Department of Molecular Virology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Scand J Immunol. 1991 May;33(5):593-605. doi: 10.1111/j.1365-3083.1991.tb02531.x.
Concomitant with induction of interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) gene expression in human tonsil cells, mitogenic stimulation induces a transient activation of cells able to effectively suppress expression of these genes. Induction of IL-2 and IFN-gamma genes largely precedes appearance of suppressor cell activity, allowing expression of both genes to occur before strong down-regulation is exerted by activated suppressor cells. Suppressive activity induced in one cell population can inhibit IL-2 and IFN-gamma gene expression in another population from the same donor. The distinct nature of suppressor cells is supported by the absence of down-regulation of IL-2 gene expression in a helper cell line, MLA-144; yet, in these cells, negative control can be expressed when active suppressor cells are introduced. Our findings support the concept that actual levels of IL-2 and IFN-gamma gene activity are regulated to a large extent by the differential kinetics of activation of suppressor cells on one hand and of cells expressing the IL-2 and IFN-gamma genes on the other.
在人扁桃体细胞中，丝裂原刺激在诱导白细胞介素-2（IL-2）和干扰素-γ（IFN-γ）基因表达的同时，会诱导能够有效抑制这些基因表达的细胞发生短暂激活。IL-2和IFN-γ基因的诱导在很大程度上先于抑制细胞活性的出现，使得这两种基因在被激活的抑制细胞进行强烈下调之前得以表达。在一个细胞群体中诱导产生的抑制活性可以抑制来自同一供体的另一个细胞群体中IL-2和IFN-γ基因的表达。辅助细胞系MLA-144中IL-2基因表达未出现下调，这支持了抑制细胞的独特性质；然而，在这些细胞中，当引入活性抑制细胞时，可以表达负调控。我们的研究结果支持这样一种概念，即IL-2和IFN-γ基因活性的实际水平在很大程度上一方面受抑制细胞激活的差异动力学调控，另一方面受表达IL-2和IFN-γ基因的细胞激活的差异动力学调控。
