McKeand J B, Knox D P, Duncan J L, Kennedy M W
Department of Veterinary Parasitology, University of Glasgow, U.K.
Int J Parasitol. 1995 Jan;25(1):95-104. doi: 10.1016/0020-7519(94)e0066-v.
Parasite preparations were examined for their ability to induce protective immunity against Dictyocaulus viviparus in guinea pigs. Dunkin-Hartley strain guinea pigs were immunised with somatic extracts of adult parasites, somatic extracts of third stage larvae or excretory/secretory (ES) products from adult parasites. The groups were immunised twice with Freund's adjuvant four weeks apart and challenged with 6000 infective L3. Significant levels of protective immunity were observed only in the adult ES-immunised animals. The antibody responses of the different groups were compared following analysis by ELISA and immunoprecipitation. To examine the protective role of antibody, guinea pigs were passively immunised with serum from animals immunised with adult ES products or serum from guinea pigs exposed to experimental D. viviparus infection. Following challenge with infective L3, lung-worm burdens of these groups were significantly lower than in guinea pigs which received normal sera. The results suggest that D. viviparus adult ES products contain protective antigens and that antibody-mediated mechanisms contribute to immune protection.
研究了寄生虫制剂诱导豚鼠对胎生网尾线虫产生保护性免疫的能力。用成年寄生虫的体细胞提取物、第三期幼虫的体细胞提取物或成年寄生虫的排泄/分泌（ES）产物对Dunkin-Hartley品系豚鼠进行免疫。这些组每隔四周用弗氏佐剂免疫两次，并用6000条感染性L3进行攻击。仅在成年ES免疫的动物中观察到显著水平的保护性免疫。通过ELISA和免疫沉淀分析后，比较了不同组的抗体反应。为了研究抗体的保护作用，用成年ES产物免疫的动物的血清或暴露于实验性胎生网尾线虫感染的豚鼠的血清对豚鼠进行被动免疫。在用感染性L3攻击后，这些组的肺线虫负荷显著低于接受正常血清的豚鼠。结果表明，胎生网尾线虫成年ES产物含有保护性抗原，且抗体介导的机制有助于免疫保护。
