Hepburn P J, Glynne-Jones E, Goddard L, Gee J M, Harper M E
Prostate Cancer Laboratory, Tenovus Cancer Research Centre, University of Wales College of Medicine, Cardiff, UK.
Histochem J. 1995 Mar;27(3):196-203.
Antibodies to assess the proliferative index of tumours are being increasingly employed together with established markers for prognostic evaluation. This study set out to compare three cell proliferation markers, Ki-67, MIB-1 and PCNA, utilizing a semi-quantitative method of assessment, in 20 human prostatic carcinomas. The streptavidin-biotin immunostaining system was used for the monoclonal antibodies MIB-1 and PCNA and an indirect immunoperoxidase assay for the monoclonal antibody Ki-67. Significant correlations were found between the expression of Ki-67 in frozen tissues and MIB-1 in formal saline-fixed wax-embedded tissues (p = 0.0003); between Ki-67 and PCNA expression in Bouin's-fixed tissues (p < or = 0.0001); and MIB-1 (formalin-saline-fixed tissues) and PCNA (Bouin's-fixed tissues) (p < or = 0.0001). A more intense nuclear staining pattern with less heterogeneity was observed for MIB-1 compared with PCNA, suggesting the antibody of choice, on formal saline-fixed tissues, is MIB-1, which closely correlated with Ki-67, a marker we have previously shown to be of prognostic value in prostatic carcinoma.
用于评估肿瘤增殖指数的抗体正越来越多地与用于预后评估的既定标志物一起使用。本研究旨在采用半定量评估方法，对20例人类前列腺癌中的三种细胞增殖标志物Ki-67、MIB-1和PCNA进行比较。链霉亲和素-生物素免疫染色系统用于单克隆抗体MIB-1和PCNA，间接免疫过氧化物酶测定用于单克隆抗体Ki-67。在冷冻组织中Ki-67的表达与在福尔马林固定石蜡包埋组织中MIB-1的表达之间发现显著相关性（p = 0.0003）；在Bouin固定组织中Ki-67与PCNA表达之间（p≤0.0001）；以及MIB-1（福尔马林-生理盐水固定组织）和PCNA（Bouin固定组织）之间（p≤0.0001）。与PCNA相比，MIB-1观察到更强烈的核染色模式且异质性更低，这表明在福尔马林固定组织上，首选抗体是MIB-1，它与Ki-67密切相关，我们之前已证明Ki-67是前列腺癌中有预后价值的标志物。
