Gillett C E, Barnes D M, Camplejohn R S
ICRF Clinical Oncology Unit, Guy's Hospital, London.
J Clin Pathol. 1993 Dec;46(12):1126-8. doi: 10.1136/jcp.46.12.1126.
The staining patterns obtained with two antibodies against proliferating cell nuclear antigen (PC10 and 19A2) and another cell cycle associated antibody (KiS1) were compared with each other and with a number of established prognostic markers of breast carcinoma. Although PC10 and 19A2 staining patterns were similar, only the latter was significantly associated with KiS1 antibody staining. These findings suggest that the two PCNA antibodies detect different epitopes. KiS1 was the only antibody to show an association with S phase fraction measured by flow cytometry (p < 0.001). It was also associated with histological grade (p = 0.003), oestrogen receptors (p = 0.045), and DNA index (p = 0.007). PC10 showed no association with any of the markers of prognosis, while 19A2 was associated with histological grade (p = 0.017) and oestrogen receptors (p = 0.043). The two PCNA antibodies do not seem to be of value in measuring proliferative activity nor do they seem to be associated with established markers of prognosis in breast cancer.
将两种抗增殖细胞核抗原的抗体（PC10和19A2）以及另一种细胞周期相关抗体（KiS1）的染色模式相互进行比较，并与一些已确立的乳腺癌预后标志物进行比较。尽管PC10和19A2的染色模式相似，但只有后者与KiS1抗体染色显著相关。这些发现表明，两种PCNA抗体检测的是不同的表位。KiS1是唯一一种与通过流式细胞术测量的S期分数相关的抗体（p < 0.001）。它还与组织学分级（p = 0.003）、雌激素受体（p = 0.045）和DNA指数（p = 0.007）相关。PC10与任何预后标志物均无关联，而19A2与组织学分级（p = 0.017）和雌激素受体（p = 0.043）相关。这两种PCNA抗体在测量增殖活性方面似乎没有价值，在乳腺癌中似乎也与已确立的预后标志物无关。
