Canvin J R, Marvin A P, Sivakumaran M, Paton J C, Boulnois G J, Andrew P W, Mitchell T J
Department of Microbiology and Immunology, University of Leicester, United Kingdom.
J Infect Dis. 1995 Jul;172(1):119-23. doi: 10.1093/infdis/172.1.119.
Mice were infected intranasally with a serotype 2 pneumococcus, a pneumolysin-negative derivative (PLN-A), or an autolysin-negative derivative (AL-2). Numbers of wild type pneumococci were seen in the lung from approximately 12 h after infection and were first detected in the blood around this time. Immunofluorescent staining of lung sections showed that pneumolysin was produced in vivo. Pneumococcal infection resulted in alteration of the composition of the blood but not the bone marrow. Some of the hematologic changes did not occur after PLN-A. PLN-A had a slower growth rate in the lung and bacteremia was delayed. AL-2 was rapidly cleared from the lungs and was not detected in the blood. These events paralleled the pattern of histology in the lung, with the severity of inflammation reduced with PLN-A and no inflammation or hematologic changes with AL-2.
将小鼠经鼻内感染2型肺炎球菌、肺炎溶血素阴性衍生物（PLN-A）或自溶素阴性衍生物（AL-2）。感染后约12小时在肺中可见野生型肺炎球菌数量，且此时首次在血液中检测到。肺切片的免疫荧光染色显示体内产生了肺炎溶血素。肺炎球菌感染导致血液成分改变，但未导致骨髓成分改变。PLN-A感染后一些血液学变化未出现。PLN-A在肺中的生长速度较慢，菌血症延迟出现。AL-2迅速从肺中清除，在血液中未检测到。这些情况与肺组织学模式相似，PLN-A使炎症严重程度降低，而AL-2感染则无炎症或血液学变化。
