Jounblat Rania, Kadioglu Aras, Mitchell Tim J, Andrew Peter W
Department of Microbiology and Immunology, University of Leicester, University Road, Leicester, LE1 9HN, UK.
Infect Immun. 2003 Apr;71(4):1813-9. doi: 10.1128/IAI.71.4.1813-1819.2003.
Pneumolysin, a multifunctional toxin produced by all clinical isolates of Streptococcus pneumoniae, is strongly implicated in the pathogenesis of pneumococcal bronchopneumonia and septicemia. Using isogenic mutant strains, we examined the effect of deletion of the cytotoxic activity or complement-activating activity of pneumolysin on bacterial growth in lungs and blood, histological changes in infected lung tissue, and the pattern of inflammatory cell recruitment. Both of the activities of pneumolysin contributed to the pathology in the lungs, as well as the timing of the onset of bacteremia. Histological changes in the lungs were delayed after infection with either mutant compared to the changes seen after infection with the wild-type pneumococcus. The complement-activating activity of pneumolysin affected the accumulation of T cells, whereas the toxin's cytolytic activity influenced neutrophil recruitment into lung tissue.
肺炎溶血素是由肺炎链球菌的所有临床分离株产生的一种多功能毒素，与肺炎球菌支气管肺炎和败血症的发病机制密切相关。我们使用同基因突变菌株，研究了肺炎溶血素细胞毒性活性或补体激活活性缺失对肺部和血液中细菌生长、感染肺组织的组织学变化以及炎症细胞募集模式的影响。肺炎溶血素的这两种活性均导致肺部病理变化以及菌血症发作的时间。与野生型肺炎球菌感染后所见的变化相比，感染任何一种突变体后肺部的组织学变化均延迟。肺炎溶血素的补体激活活性影响T细胞的聚集，而该毒素的溶细胞活性则影响中性粒细胞向肺组织的募集。
