Wakabayashi K, Matsuzaki H
Department of Orthopaedic Surgery, Nihon University School of Medicine, Tokyo.
Nihon Seikeigeka Gakkai Zasshi. 1995 May;69(5):311-21.
Disc grafting is a new surgical procedure that may be suitable for preserving mobility of the spine. This experimental study was designed to determine a method of favorably preserving a disc prior to allografting. The lumber vertebrae of adult mongrel dogs were aseptically excised, regarded as the vertebral body-disc unit, and preserved at -80 degrees C, or at 4 degrees C. In the preservation at 4 degrees C, UW solution, Euro-Collins solution, EP-II solution, Ham's F-12, or physiological saline solution was used for preservation. Isotopes were used for determining the activities of the disc cells. In the preservation at -80 degrees C, it was difficult to maintain the activity regardless of the preservation period. There was no difference among these preservative solutions in the preservation at 4 degrees C on the nucleus pulposus, but the 2-day preservation in UW solution provided the best results in terms of the annulus fibrosus. Based on the results of these experiments, at -80 degrees C it was difficult to maintain the activity of disc cells while at 4 degrees C the UW solution was the most effective for preserving the anulus fibrosus. However the period of preservation was very short, and a new method to enable longer periods of preservation should be developed.
椎间盘移植是一种可能适用于保持脊柱活动度的新型外科手术。本实验研究旨在确定一种在同种异体移植前良好保存椎间盘的方法。成年杂种犬的腰椎被无菌切除，视为椎体 - 椎间盘单元，并保存在 -80℃或4℃。在4℃保存时，使用UW溶液、Euro - Collins溶液、EP - II溶液、Ham's F - 12或生理盐水溶液进行保存。使用同位素来测定椎间盘细胞的活性。在 -80℃保存时，无论保存时间长短，都难以维持细胞活性。在4℃保存时，这些保存液对髓核的保存效果没有差异，但就纤维环而言，在UW溶液中保存2天效果最佳。基于这些实验结果，在 -80℃难以维持椎间盘细胞的活性，而在4℃时，UW溶液对保存纤维环最有效。然而，保存时间非常短，应该开发一种能够实现更长保存时间的新方法。
