Akazawa C, Shigemoto R, Bessho Y, Nakanishi S, Mizuno N
Department of Morphological Brain Science, Kyoto University, Japan.
J Comp Neurol. 1994 Sep 1;347(1):150-60. doi: 10.1002/cne.903470112.
Five N-methyl-D-aspartate (NMDA) receptor subunits have been identified thus far: NR1, NR2A, NR2B, NR2C, and NR2D. Here, we have analyzed the expression patterns of mRNAs for the NMDA receptor subunits in the developing and adult rats by in situ hybridization. The developmental changes of the expression patterns were most salient in the cerebellum. In the external granular layer, hybridization signals of mRNAs for NR1, NR2A, NR2B, and NR2C appeared by postnatal day 3, but no NR2D mRNA was expressed at any developmental stage examined. The NR1 mRNA was expressed in all cerebellar neurons at all developmental stage examined. The NR1 mRNA was expressed in all cerebellar neurons at all developmental stages examined. The signals for the NR2A mRNA appeared in Purkinje cells and granule cells during the second postnatal week. The signals for the NR2B mRNA in granule cells were seen transiently during the first 2 weeks after birth. The signals for NR2C mRNA appeared in granule cells and glial cells during the second postnatal week. The signals for NR2D mRNA appeared transiently in Purkinje cells during the first 8 postnatal days; in adult rats, these were seen in stellate and Golgi cells. In the cerebellar nuclei, mRNAs for NR1, NR2A, NR2B, and NR2D were more or less expressed on postnatal day 0, while expression signals for the NR2C mRNA were first detected in postnatal day 14. Thus, the most conspicuous changes of expression patterns were observed in the cerebellar cortex during the first 2 weeks after birth, when development and maturation of the cerebellum proceed most rapidly.
到目前为止，已鉴定出五种N-甲基-D-天冬氨酸（NMDA）受体亚基：NR1、NR2A、NR2B、NR2C和NR2D。在此，我们通过原位杂交分析了发育中和成年大鼠中NMDA受体亚基mRNA的表达模式。表达模式的发育变化在小脑中最为显著。在颗粒层外层，出生后第3天出现NR1、NR2A、NR2B和NR2C mRNA的杂交信号，但在所检查的任何发育阶段均未表达NR2D mRNA。在所有检查的发育阶段，NR1 mRNA在所有小脑神经元中均有表达。出生后第二周，Purkinje细胞和颗粒细胞中出现NR2A mRNA的信号。出生后前两周，颗粒细胞中短暂出现NR2B mRNA的信号。出生后第二周，颗粒细胞和神经胶质细胞中出现NR2C mRNA的信号。出生后前8天，Purkinje细胞中短暂出现NR2D mRNA的信号；在成年大鼠中，在星状细胞和高尔基细胞中可见这些信号。在小脑核中，出生后第0天或多或少表达NR1、NR2A、NR2B和NR2D的mRNA，而NR2C mRNA的表达信号在出生后第14天首次检测到。因此，在出生后前两周小脑皮质中观察到表达模式最明显的变化，此时小脑的发育和成熟最为迅速。
