Piekoszewski W, Chow F S, Jusko W J
Department of Pharmaceutics, State University of New York at Buffalo 14260.
Immunopharmacol Immunotoxicol. 1994 Aug;16(3):389-401. doi: 10.3109/08923979409007100.
A whole blood lymphocyte proliferation assay was compared to a standard method requiring the isolation of lymphocytes from blood. Both methods were used to measure inhibition of proliferative responses of phytohaemagglutinin (PHA1)-stimulated human peripheral blood lymphocytes by tacrolimus (FK 506(1)), cyclosporine A (CsA1), rapamycin (RA1), dexamethasone (DEX1), prednisolone (PR1), and methylprednisolone (MP1). Three of the drugs studied (FK 506, CsA, and DEX) yielded similar IC50 values with both methods. The whole blood proliferation assay produced modestly lower IC50 values for RA, PR and MP. The whole blood lymphocyte proliferation method is simple and can be used when only limited volumes of blood can be obtained or isolation of cells gives unsatisfactory yields.
将全血淋巴细胞增殖试验与一种需要从血液中分离淋巴细胞的标准方法进行了比较。两种方法都用于测量他克莫司（FK 506(1)）、环孢素A（CsA1）、雷帕霉素（RA1）、地塞米松（DEX1）、泼尼松龙（PR1）和甲泼尼龙（MP1）对植物血凝素（PHA1）刺激的人外周血淋巴细胞增殖反应的抑制作用。所研究的三种药物（FK 506、CsA和DEX）在两种方法中产生的半数抑制浓度（IC50）值相似。全血增殖试验对RA、PR和MP产生的IC50值略低。全血淋巴细胞增殖方法简单，当只能获得有限体积的血液或细胞分离产量不理想时可以使用。
