Mizutani H, Hayashi T, Nouchi N, Ohyanagi S, Hashimoto K, Shimizu M, Suzuki K
Department of Dermatology, Mie University, Faculty of Medicine, Japan.
J Invest Dermatol. 1994 Dec;103(6):825-8. doi: 10.1111/1523-1747.ep12413560.
In an immunohistopathologic study using two monoclonal antibodies that recognize independent epitopes on human thrombomodulin, we detected expression of thrombomodulin on Malpighian-layer keratinocytes, but not on the basal or upper granular-layer keratinocytes. Western blotting and thrombomodulin-specific enzyme-linked immunosorbent assay performed on both shaved skin and cultured SV40-transformed keratinocytes showed the expression of full-length thrombomodulin, indistinguishable from endothelial thrombomodulin. Thrombomodulin was expressed by keratinocytes in 1 mM calcium but not in reduced calcium. A functional assay for thrombomodulin revealed that epidermal-derived thrombomodulin possessed cofactor activity for thrombin-catalyzed protein C activation. The limited pattern of expression of thrombomodulin in epidermis implies that this molecule may be a differentiation marker of keratinocytes in addition to being a potent anticoagulant in the skin.
在一项免疫组织病理学研究中，我们使用两种识别人类血栓调节蛋白上独立表位的单克隆抗体，检测到马尔皮基层角质形成细胞上有血栓调节蛋白表达，但基底层或颗粒层上层角质形成细胞上未检测到。对剃毛皮肤和培养的SV40转化角质形成细胞进行的蛋白质印迹法和血栓调节蛋白特异性酶联免疫吸附测定显示，全长血栓调节蛋白有表达，与内皮血栓调节蛋白无差异。角质形成细胞在1 mM钙环境中表达血栓调节蛋白，而在低钙环境中不表达。血栓调节蛋白的功能测定显示，表皮来源的血栓调节蛋白具有凝血酶催化蛋白C活化的辅因子活性。血栓调节蛋白在表皮中的有限表达模式表明，该分子除了是皮肤中的一种有效抗凝剂外，可能还是角质形成细胞的分化标志物。
