分子通过皮肤转运的直接成像。

Direct imaging of molecular transport through skin.

作者信息

Scott E R, Phipps J B, White H S

机构信息

Medtronic, Inc., Brooklyn Center, Minnesota.

出版信息

J Invest Dermatol. 1995 Jan;104(1):142-5. doi: 10.1111/1523-1747.ep12613661.

DOI:10.1111/1523-1747.ep12613661

PMID:7798633

Abstract

Scanning electrochemical microscopy (SECM) was used to image spatial variations in the molecular flux of Fe(CN)6(-4) across excised hairless and nude mouse skin. The SECM response is specific to electroactive molecules, allowing selective imaging of the flux of Fe(CN)6(-4) in multicomponent ionic solutions. Quantitative SECM image analysis demonstrated that 40% to 60% of the total Fe(CN)6(-4) flux occurred through appendages in the skin. SECM analysis of skin samples exposed to a known transport enhancer, sodium dodecylsulfate, indicated that the increase in the ion transport rate occurred exclusively in nonporous skin tissue.

摘要

扫描电化学显微镜（SECM）用于成像Fe(CN)₆⁴⁻跨切除的无毛和裸鼠皮肤的分子通量的空间变化。SECM响应对电活性分子具有特异性，能够对多组分离子溶液中Fe(CN)₆⁴⁻的通量进行选择性成像。定量SECM图像分析表明，总Fe(CN)₆⁴⁻通量的40%至60%通过皮肤附属器发生。对暴露于已知转运增强剂十二烷基硫酸钠的皮肤样本进行的SECM分析表明，离子转运速率的增加仅发生在无孔皮肤组织中。