Valdez F, Hernández M, Govezensky T, Fragoso G, Sciutto E
Departamento de Inmunología, Universidad Nacional Autonoma de México, México, D.F.
J Parasitol. 1994 Dec;80(6):931-6.
Cross immunity between Taenia solium and Taenia crassiceps parasites points to T. crassiceps cysticercosis as a convenient model to test promising antigens aimed at the development of a vaccine against T. solium cysticercosis. Since total antigens from T. crassiceps metacestodes induce significant levels of protection in pigs against T. solium cysticercosis, we initiated this work to identify the most interesting antigens involved in protection. Twelve different antigen fractions isolated from T. crassiceps cysticerci were evaluated with respect to their capacity to induce resistance against a challenge with 10 T. crassiceps cysticerci in male BALB/cAnN mice. Mice were intraperitoneally immunized with 2 doses of each antigen, 5 or 15 micrograms per mouse. The 12 antigen fractions were classified as protecting (200, 123, 74, 66, 56, 40-50, 27 and 8-14 kDa), facilitating (220-205 kDa), or irrelevant (150-160, 93, 108 kDa), according to their effect on the parasite load. The 3 most promising antigen fractions were reevaluated via subcutaneous immunization with Freund's complete adjuvant. A high level of protection was obtained when antigen fractions of 56, 66, and 74 kDa were used together. Interestingly, antigens with similar molecular weights were also detected in early steps of differentiation in T. solium cysticercosis. These observations may be helpful in the development of a synthetic or a recombinant vaccine against cysticercosis.
猪带绦虫和肥胖带绦虫寄生虫之间的交叉免疫表明，肥胖带绦虫囊尾蚴病是一个方便的模型，可用于测试有前景的抗原，以开发针对猪带绦虫囊尾蚴病的疫苗。由于肥胖带绦虫中绦期的总抗原能在猪体内诱导出对猪带绦虫囊尾蚴病的显著保护水平，我们开展了这项工作，以鉴定参与保护作用的最具吸引力的抗原。从肥胖带绦虫囊尾蚴中分离出12种不同的抗原组分，评估它们诱导雄性BALB/cAnN小鼠抵抗10个肥胖带绦虫囊尾蚴攻击的能力。小鼠腹腔注射每种抗原2剂，每只小鼠5或15微克。根据它们对寄生虫负荷的影响，这12种抗原组分被分类为具有保护作用（200、123、74、66、56、40 - 50、27和8 - 14 kDa）、促进作用（220 - 205 kDa）或无作用（150 - 160、93、108 kDa）。通过用弗氏完全佐剂皮下免疫，对3种最有前景的抗原组分进行了重新评估。当一起使用56、66和74 kDa的抗原组分时，获得了高水平的保护。有趣的是，在猪带绦虫囊尾蚴病分化的早期阶段也检测到了分子量相似的抗原。这些观察结果可能有助于开发针对囊尾蚴病的合成疫苗或重组疫苗。
