Onagbesan O M, Gullick W, Woolveridge I, Peddie M J
Department of Physiology and Pharmacology, University of Southampton, UK.
J Reprod Fertil. 1994 Sep;102(1):147-53. doi: 10.1530/jrf.0.1020147.
The purpose of this study was to determine the presence of epidermal growth factor receptor and its potential ligands epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha) in the tissues of the maturing follicles in the ovary of laying ISA-Brown hens using peptide-specific immunohistochemical methods. Cryostat sections, 6-8 microns thick, were made from fresh-frozen tissues of F1-F4 (largest to fourth largest) and large white follicles and they were immunostained for epidermal growth factor receptor, epidermal growth factor or transforming growth factor alpha using specific polyclonal antibodies. The EGF receptor and both ligands were detected in the granulosa, theca interna and theca externa layers of the follicles. The EGF receptor was localized both in the plasma membrane and cytoplasm of all cell types. EGF was predominantly cytosolic, whereas TGF-alpha was found in the plasma membranes and perinuclear areas of all cell types. The concentration of the receptor and both ligands decreased with follicular maturation. This observation is consistent with our previous observation that the response to EGF and TGF-alpha decreases as follicles mature, and thus provides further evidence that the receptor or the ligands may have a regulatory role in avian ovarian function.
本研究的目的是采用肽特异性免疫组织化学方法，确定产蛋ISA - 褐壳蛋鸡卵巢中成熟卵泡组织中表皮生长因子受体及其潜在配体表皮生长因子（EGF）和转化生长因子α（TGF -α）的存在情况。从F1 - F4（最大至第四大）和大白卵泡的新鲜冷冻组织制备6 - 8微米厚的冰冻切片，并用特异性多克隆抗体对其进行表皮生长因子受体、表皮生长因子或转化生长因子α的免疫染色。在卵泡的颗粒层、卵泡内膜层和卵泡外膜层中检测到了EGF受体及其两种配体。EGF受体定位于所有细胞类型的质膜和细胞质中。EGF主要存在于细胞质中，而TGF -α存在于所有细胞类型的质膜和核周区域。受体及其两种配体的浓度随卵泡成熟而降低。这一观察结果与我们之前的观察结果一致，即随着卵泡成熟，对EGF和TGF -α的反应降低，从而进一步证明受体或配体可能在禽类卵巢功能中具有调节作用。
