Scurry J P, Hamand K A, Astley S B, Leake R E, Wells M
Department of Pathology, Mercy Hospital for Women, East Melbourne, Victoria, England.
Pathology. 1994 Apr;26(2):130-3. doi: 10.1080/00313029400169351.
This study provides a valuable insight into the localization of growth factors in paraffin sections of human ovarian tissue. Antibodies to epidermal growth factor (EGF), transforming growth factors alpha and beta (TGF alpha and beta) and epidermal growth factor receptor (EGFR) were applied to paraffin sections of 16 cases of formalin-fixed normal or benignly abnormal ovarian tissue. All growth factor antibodies reacted with theca, but not granulosa cells, whilst the antibody to EGFR reacted with both types of follicular cells and was weakly reactive in ovarian stroma. There were no discernible qualitative changes in reactivity during the follicular cycle. These immunohistochemical findings generally support previously published molecular and biochemical data from tissue culture. One exception is in the observation of immunoreactivity to EGF in theca and granulosa cells. This may be due to differences in sensitivity of the methods in use. The possibility of a cross-reaction of the anti-EGF antibody with TGF alpha is also discussed. This study provides evidence for both paracrine and autocrine roles for growth factors in folliculogenesis.
本研究为了解生长因子在人卵巢组织石蜡切片中的定位提供了有价值的见解。将针对表皮生长因子（EGF）、转化生长因子α和β（TGFα和TGFβ）以及表皮生长因子受体（EGFR）的抗体应用于16例经福尔马林固定的正常或良性异常卵巢组织的石蜡切片。所有生长因子抗体均与卵泡膜细胞反应，但不与颗粒细胞反应，而抗EGFR抗体则与两种卵泡细胞均有反应，且在卵巢基质中反应较弱。在卵泡周期中，反应性没有明显的定性变化。这些免疫组化结果总体上支持先前发表的来自组织培养的分子和生化数据。一个例外是观察到卵泡膜细胞和颗粒细胞对EGF有免疫反应性。这可能是由于所使用方法的敏感性不同所致。还讨论了抗EGF抗体与TGFα交叉反应的可能性。本研究为生长因子在卵泡发生中的旁分泌和自分泌作用提供了证据。
