通过聚合酶链反应从日本斑点热群立克次体病患者血液样本中检测病原体DNA。

Detection of DNA of causative agent of spotted fever group rickettsiosis in Japan from the patient's blood sample by polymerase chain reaction.

作者信息

Tange Y, Matsumoto M, Okada T, Hasegawa H, Kobayashi Y

机构信息

First Department of Internal Medicine, School of Medicine, Ehime University, Japan.

出版信息

Microbiol Immunol. 1994;38(8):665-8. doi: 10.1111/j.1348-0421.1994.tb01837.x.

DOI:10.1111/j.1348-0421.1994.tb01837.x

PMID:7799840

Abstract

The polymerase chain reaction (PCR) was applied for the etiological diagnosis of spotted fever group (SFG) rickettsiosis in Japan. Nucleotide primers derived from the 17-kDa antigen gene of Rickettsia rickettsii primed a rickettsia-specific 246-base-pair product for all of the Katayama, Abe, Misaka and Kojima strains, which we had isolated previously. Moreover, we were able to detect the same product by PCR amplification from the peripheral blood of a patient in the acute stage of the illness. The PCR method is considered to be useful for rapid etiological diagnosis of SFG rickettsiosis in Japan.

摘要

聚合酶链反应（PCR）被应用于日本斑点热群（SFG）立克次体病的病原学诊断。从立氏立克次体17-kDa抗原基因衍生的核苷酸引物，能扩增出针对我们之前分离的所有片山、阿部、三坂和小岛菌株的立克次体特异性246碱基对产物。此外，我们能够通过PCR扩增从一名疾病急性期患者的外周血中检测到相同产物。PCR方法被认为对日本SFG立克次体病的快速病原学诊断有用。