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[Standardization of an inhibition ELISA technique for the serologic diagnosis of coxsackie virus group B infections].

作者信息

Balmaseda Hechavarría A, Más Lago P, Vázquez Ramudo S, Comellas Fernández M M

机构信息

Instituto de Medicina Tropical Pedro Kouri.

出版信息

Rev Cubana Med Trop. 1993;45(1):63-6.

PMID:7800894
Abstract

An inhibition ELISA technic to detect antibodies aimed at Coxsackie B viruses was standardized. The method was type-specific since it could detect antibodies to 4 Coxsackie B serotypes (it wasn't tried on serotypes B2 and B4 because the strains were not available). The comparison with the neutralization technic showed 85% coincidence, 91% sensitivity and 82% specificity. All the reactives used in the assay were produced in our laboratory.

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