del Rosario Zuñiga M, Reichardt J, Braun W, Weber B, Doerr H W
Klinikum, Johann Wolfgang Goethe-Universität, Frankfurt, Germany.
Acta Virol. 1993 Feb;37(1):1-10.
We report the application of a modified Western blot (WB) micromethod basically relying on a diffusion-blotting technique (Modi-blot) combined with an immunological detection system using monoclonal antibodies and a biotinavidin amplification step for the detection of IgM antibodies against coxsackieviruses B1 (CBV1), CBV2 and CBV4. Fifty-one adult patients with clinical signs of coxsackievirus B infection (e.g. myocarditis and meningitis) were investigated. The test revealed a total of 31 (60%) IgM positives. The majority of IgM antibodies were group reactive (22/31). Type-specific antibodies could be recognized in 9 cases (3 with CBV1, 5 with CBV2 and 1 with CBV4). The highest rate of antibody prevalence was found in sera from patients with acute meningitis (12/14). Controls [healthy adults (n = 13) and individuals with other infections (n = 13)] were all negative for specific IgM against coxsackievirus B1, B2 and B4. Further WB tests of 8 IgM positive specimens with coxsackievirus B4 revealed specific IgA responses in all cases, reinforcing the evidence for a recent infection. In addition, the patterns of IgG antibody subclasses, also investigated in this group, showed a clear predominance of specific IgG1 and IgG3 antibodies.
我们报告了一种改良的蛋白质印迹法（WB）微量方法的应用，该方法主要基于扩散印迹技术（改良印迹法），并结合使用单克隆抗体的免疫检测系统和生物素-抗生物素蛋白扩增步骤，用于检测抗柯萨奇病毒B1（CBV1）、CBV2和CBV4的IgM抗体。对51例有柯萨奇病毒B感染临床症状（如心肌炎和脑膜炎）的成年患者进行了调查。检测发现共有31例（60%）IgM呈阳性。大多数IgM抗体具有群反应性（22/31）。在9例中可识别出型特异性抗体（3例针对CBV1，5例针对CBV2，1例针对CBV4）。抗体流行率最高的是急性脑膜炎患者的血清（12/14）。对照组[健康成年人（n = 13）和其他感染患者（n = 13）]针对柯萨奇病毒B1、B2和B4的特异性IgM均为阴性。对8份抗柯萨奇病毒B4的IgM阳性标本进行的进一步WB检测显示，所有病例均有特异性IgA反应，进一步证明了近期感染。此外，在该组中还研究了IgG抗体亚类的模式，结果显示特异性IgG1和IgG3抗体明显占优势。
