Tenner-Racz K, von Stemm A M, Gühlk B, Schmitz J, Racz P
Bernhard-Nocht Institute for Tropical Medicine, Hamburg, Germany.
Res Virol. 1994 May-Aug;145(3-4):177-82. doi: 10.1016/s0923-2516(07)80020-3.
Numerous data demonstrate that lymphoid tissues represent a major HIV reservoir and sites of virus replication. To gain more information about the phenotype of cells in which HIV replicates in the lymphoid tissue, we applied HIV RNA hybridization technique in combination with immunohistochemistry on 10 lymph nodes and 2 lymphoepithelial cysts (LEC) of the parotid gland. In addition, to determine whether or not follicular dendritic cells (FDC) are important targets for HIV, we also analysed isolated and sorted FDC from 4 lymph nodes. The tissues showed follicular hyperplasia and heavy RNA signals in the germinal centres (GC), where diffuse labelling in the light zone as well as single cells with > 50 silver grains were present. The extrafollicular parenchyma contained only a few productively infected cells. The diffuse labelling was intimately related to the FDC network. However, electron microscopic examination and in situ hybridization on isolated FDC did not provide convincing evidence for virus replication in these cells. Virus-producing cells in the GC were almost exclusively CD45RO+, CD57- T cells. Macrophages with HIV RNA were mainly found in LEC of the parotid gland. S-100+ interdigitating cells of the T-dependent zone were HIV RNA-. However, some large S-100+ cells in the squamous epithelium covering the LEC or lying subepithelial did express HIV RNA. These findings suggest that local factors (cytokines?) in the lymphoid tissue probably influence the expression of HIV.
大量数据表明，淋巴组织是主要的HIV储存库和病毒复制场所。为了获取更多关于HIV在淋巴组织中复制的细胞表型的信息，我们将HIV RNA杂交技术与免疫组织化学相结合，应用于10个淋巴结和2个腮腺淋巴上皮囊肿（LEC）。此外，为了确定滤泡树突状细胞（FDC）是否是HIV的重要靶细胞，我们还分析了从4个淋巴结中分离和分选的FDC。组织显示生发中心（GC）有滤泡增生和强烈的RNA信号，在亮区有弥漫性标记以及存在>50个银颗粒的单个细胞。滤泡外实质仅含有少数有生产性感染的细胞。弥漫性标记与FDC网络密切相关。然而，对分离的FDC进行电子显微镜检查和原位杂交并未提供这些细胞中病毒复制的确凿证据。GC中的病毒产生细胞几乎全是CD45RO+、CD57-T细胞。带有HIV RNA的巨噬细胞主要见于腮腺的LEC。T依赖区的S-100+交错突细胞HIV RNA阴性。然而，覆盖LEC或位于上皮下的鳞状上皮中的一些大的S-100+细胞确实表达HIV RNA。这些发现表明，淋巴组织中的局部因素（细胞因子？）可能影响HIV的表达。
