Persidsky Y, Berger S, Gendrault J L, Steffan A M, Royer C, Hurtrel B, Stutte H J, Kirn A, Aubertin A M
Unité INSERM 74 et Institut de Virologie de la Faculté de Médecine, Strasbourg, France.
Res Virol. 1994 May-Aug;145(3-4):229-37. doi: 10.1016/s0923-2516(07)80027-6.
The livers of 21 rhesus monkeys inoculated with SIVmac251 were examined at 4 days to 39 months after infection. SIV antigens were detected in the cytoplasm of Kupffer cells (KC), macrophages and lymphocytes in two-thirds of the livers tested. The number of cells containing viral proteins substantially increased during the development of the disease, and KC were the main cell type displaying SIV proteins at an advanced stage of infection. Mature and immature lentiviral particles were found in cytoplasmic vacuoles or associated with worm-like structures in KC, indicating that SIV replication could occur within resident liver macrophages. Another sign of the permissiveness of KC was the formation of multinucleated giant cells within the hepatic sinusoids. Some of these cells containing 3-6 nuclei still retained ultrastructural features of KC. Most of them contained a high quantity of viral particles. Numerous lymphocytes displaying signs of apoptosis were taken up by KC, especially at the beginning of infection. Hyperplasia and hypertrophy of KC were noted in the course of SIV disease in the liver. The present data indicate that KC can be infected in vivo and may serve as a reservoir for SIV during the progression of the disease.
对21只接种了SIVmac251的恒河猴肝脏在感染后4天至39个月进行检查。在三分之二的受试肝脏中，库普弗细胞（KC）、巨噬细胞和淋巴细胞的细胞质中检测到SIV抗原。在疾病发展过程中，含有病毒蛋白的细胞数量大幅增加，在感染后期，KC是显示SIV蛋白的主要细胞类型。在细胞质空泡中或与KC中的蠕虫样结构相关处发现了成熟和未成熟的慢病毒颗粒，这表明SIV可在驻留肝巨噬细胞内复制。KC易感性的另一个迹象是肝血窦内多核巨细胞的形成。其中一些含有3 - 6个核的细胞仍保留着KC的超微结构特征。它们中的大多数含有大量病毒颗粒。特别是在感染初期，大量显示凋亡迹象的淋巴细胞被KC吞噬。在肝脏SIV疾病过程中，观察到KC的增生和肥大。目前的数据表明，KC可在体内被感染，并且在疾病进展过程中可能作为SIV的储存库。
