Prehm P, Stirm S, Jann B, Jann K, Boman H G
Eur J Biochem. 1976 Jul 1;66(2):369-77. doi: 10.1111/j.1432-1033.1976.tb10526.x.
The lipopolysaccharides of ampicillin-resistant cell-wall-defective mutants of Escherichia coli K-12 were analyzed. From their lipopolysaccharides the respective core oligosaccharides were obtained. Following dephosphorylation,the core oligosaccharides were methylated and analyzed by gas chromatography/mass spectrometry. From core-defective mutants substructures of the K-12 core were obtained. Analysis of the lipopolysaccharide preparations from wild-type K-12 indicated the presence of several core structures with different degrees of completion. The lipopolysaccharide preparation was degraded and the oligosaccharide mixture was partially resolved by gel filtration chromatography. Methylation, gas chromatography and mass spectrometry of the oligosaccharides permitted the tentative formulation of the K-12 core structure. Alternative interpretations for this heterogeneity are discussed.
对大肠杆菌K-12氨苄青霉素抗性细胞壁缺陷型突变体的脂多糖进行了分析。从其脂多糖中获得了各自的核心寡糖。去磷酸化后，对核心寡糖进行甲基化并通过气相色谱/质谱分析。从核心缺陷型突变体中获得了K-12核心的亚结构。对野生型K-12脂多糖制剂的分析表明存在几种不同完成程度的核心结构。将脂多糖制剂降解，通过凝胶过滤色谱法对寡糖混合物进行部分分离。寡糖的甲基化、气相色谱和质谱分析允许初步确定K-12核心结构。讨论了这种异质性的其他解释。
