通过聚合酶链反应揭示澳大利亚马匹福尔马林固定的肉瘤中牛乳头瘤病毒的基因组序列。
Genomic sequences of bovine papillomaviruses in formalin-fixed sarcoids from Australian horses revealed by polymerase chain reaction.
作者信息
Bloch N, Breen M, Spradbrow P B
机构信息
Department of Veterinary Pathology, University of Queensland, Australia.
出版信息
Vet Microbiol. 1994 Jul;41(1-2):163-72. doi: 10.1016/0378-1135(94)90145-7.
Seventy six formalin-fixed paraffin-embedded sarcoids from 62 Australian horses, collected over a ten year period, were examined for the presence of genomic sequences from bovine papillomavirus 1 and 2 (BPV1, BPV2) with the polymerase chain reaction (PCR). Sequences that could be amplified by primers specific for BPV1 and BPV2 were present in 56 of the 76 sarcoids (73%). A restriction site present in BPV1 and absent from BPV2 was detected in 28 of 34 amplified products that were treated with endonuclease.
在十年期间收集的来自62匹澳大利亚马的76个福尔马林固定石蜡包埋的肉样瘤，采用聚合酶链反应（PCR）检测牛乳头瘤病毒1型和2型（BPV1、BPV2）的基因组序列。在76个肉样瘤中的56个（73%）中存在可被BPV1和BPV2特异性引物扩增的序列。在用核酸内切酶处理的34个扩增产物中的28个中检测到BPV1中存在而BPV2中不存在的一个限制性位点。
Zotero 插件