Bloch N, Breen M, Spradbrow P B
Department of Veterinary Pathology, University of Queensland, Australia.
Vet Microbiol. 1994 Jul;41(1-2):163-72. doi: 10.1016/0378-1135(94)90145-7.
Seventy six formalin-fixed paraffin-embedded sarcoids from 62 Australian horses, collected over a ten year period, were examined for the presence of genomic sequences from bovine papillomavirus 1 and 2 (BPV1, BPV2) with the polymerase chain reaction (PCR). Sequences that could be amplified by primers specific for BPV1 and BPV2 were present in 56 of the 76 sarcoids (73%). A restriction site present in BPV1 and absent from BPV2 was detected in 28 of 34 amplified products that were treated with endonuclease.
在十年期间收集的来自62匹澳大利亚马的76个福尔马林固定石蜡包埋的肉样瘤，采用聚合酶链反应（PCR）检测牛乳头瘤病毒1型和2型（BPV1、BPV2）的基因组序列。在76个肉样瘤中的56个（73%）中存在可被BPV1和BPV2特异性引物扩增的序列。在用核酸内切酶处理的34个扩增产物中的28个中检测到BPV1中存在而BPV2中不存在的一个限制性位点。
