牛分枝杆菌卡介苗培养滤液的抗原特性分析

Antigenic characterization of Mycobacterium bovis BCG culture filtrate.

作者信息

Gupta V K, Ram G C, Bansal M P

机构信息

Immunology Section, Indian Veterinary Research Institute, Izatnagar.

出版信息

Vet Microbiol. 1994 Aug 15;41(4):345-53. doi: 10.1016/0378-1135(94)90030-2.

DOI:10.1016/0378-1135(94)90030-2

PMID:7801534

Abstract

Mycobacterium bovis BCG culture filtrate, on gelfiltration chromatography revealed four prominent regions. Of these 4, region II possessed the highest antigenic reactivity by enzyme-linked immunosorbent assay (ELISA) and delayed type hypersensitivity (DTH) skin testing in guinea pigs. On anion-exchange chromatography, region II was resolved into 5 prominent fractions of which fraction II was found to have the highest antigenic reactivity. On sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE), M. bovis BCG culture filtrate revealed 11 structural polypeptides. Fraction II yielded a homogenous polypeptide of 15.3 kDa molecular weight. All fractions cross-reacted with various mycobacteria used in this study except a 15.3 kDa polypeptide which was specific to M. bovis BCG.

摘要

牛分枝杆菌卡介苗培养滤液经凝胶过滤层析显示出四个显著区域。在这4个区域中，区域II通过酶联免疫吸附测定（ELISA）和豚鼠迟发型超敏反应（DTH）皮肤试验具有最高的抗原反应性。在阴离子交换层析中，区域II被分离为5个显著的组分，其中组分II被发现具有最高的抗原反应性。在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）中，牛分枝杆菌卡介苗培养滤液显示出11种结构多肽。组分II产生了一种分子量为15.3 kDa的均一多肽。除了一种对牛分枝杆菌卡介苗特异的15.3 kDa多肽外，所有组分都与本研究中使用的各种分枝杆菌发生交叉反应。