Dumaswala U J, Bentley N L, Greenwalt T J
Hoxworth Blood Center, University of Cincinnati Medical Center, Cincinnati, Ohio.
Vox Sang. 1994;67(2):139-43. doi: 10.1111/j.1423-0410.1994.tb01648.x.
The purpose of the present study was to determine whether a hypotonic additive containing a low concentration of glycerol as a membrane permeable solute would improve the liquid storage of red blood cells (RBCs). Packed RBCs were stored either with 200 ml of an experimental additive solution, EAS 25, containing (mM): glycerol 150, adenine 2, glucose 110, mannitol 55, and NaCl 50, or with 100 ml/unit of a conventional additive solution Adsol. The results show that the adenosine triphosphate values, hemolysis, potassium leakage, and the morphology scores of RBCs were significantly better with EAS 25 than with Adsol up to 84 days of storage. The ATP values were significantly different only after the first 42 days of storage. The mean corpuscular volumes (MCVs) of the RBCs were significantly higher throughout in the experimental additive accompanied by decreased microvesiculation as compared to Adsol. The total microvesicle membrane protein shed by 100 ml of RBCs was 47.92 +/- 12.31 mg in Adsol and 18.96 +/- 5.49 mg in EAS 25 (p < 0.001). The larger MCVs of the RBCs in EAS 25 may have a favorable effect on maintaining membrane integrity by decreasing the loss of membrane by microvesiculation.
本研究的目的是确定一种含有低浓度甘油作为膜通透性溶质的低渗添加剂是否能改善红细胞（RBC）的液体储存。浓缩红细胞分别储存于200 ml含（毫摩尔）：甘油150、腺嘌呤2、葡萄糖110、甘露醇55和氯化钠50的实验添加剂溶液EAS 25中，或100 ml/单位的传统添加剂溶液Adsol中。结果显示，在长达84天的储存期内，EAS 25组红细胞的三磷酸腺苷值、溶血、钾泄漏和形态学评分均显著优于Adsol组。仅在储存的前42天后，三磷酸腺苷值才有显著差异。与Adsol相比，在整个实验添加剂中，红细胞的平均红细胞体积（MCV）显著更高，同时微泡形成减少。100 ml红细胞在Adsol中释放的总微泡膜蛋白为47.92 +/- 12.31 mg，在EAS 25中为18.96 +/- 5.49 mg（p < 0.001）。EAS 25中红细胞较大的MCV可能通过减少微泡形成导致的膜损失对维持膜完整性产生有利影响。
