Dumaswala U J, Rugg N, Greenwalt T J
Hoxworth Blood Center, University of Cincinnati Medical Center, OH 45267-0055.
Vox Sang. 1994;67(3):255-9. doi: 10.1111/j.1423-0410.1994.tb01247.x.
Previous studies have demonstrated that an additive solution containing ammonium chloride (NH4+) and phosphate (Pi) in addition to adenine, glucose and mannitol would support red blood cell (RBC) in vitro characteristics and in vivo 24-hour viability after storage for 9 weeks. The purpose of the present study was to determine if NH4+ generated by the action of glutaminase on glutamine could be substituted for added NH4+ salts. Packed RBCs were stored with equal volumes of adenine, glucose, mannitol, and citrate containing additive solutions with 10 mM glutamine (EAS 31) or with 10 mM glutamine and either 10 (EAS 36) or 20 mM (EAS 37) Pi. One aliquot was stored with Adsol. The mean ATP levels of the RBCs stored in the glutamine plus phosphate EASs were 132 (10 mM Pi) and 144% (20 mM Pi) of the initial levels at 28 days, and at 84 days remained at 48 and 56%, respectively. The ATP levels of the RBC stored in Adsol were 105 and 25% at 28 and 84 days of storage, respectively. Percentage hemolysis and vesiculation was significantly lower (p < 0.01) for RBCs stored in glutamine and glutamine plus phosphate as compared to RBCs stored in Adsol. The levels of NH4+ were 22 to 34% higher in the EASs than in Adsol at the end of 84 days of storage, suggesting that glutamine is broken down by glutaminase to generate NH4+. The mean corpuscular volumes (MCVs) of RBCs in EASs 36 and 37 were substantially higher than in Adsol throughout the course of storage (p < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
先前的研究表明，一种除了含有腺嘌呤、葡萄糖和甘露醇外，还含有氯化铵（NH4+）和磷酸盐（Pi）的添加剂溶液，能够维持红细胞（RBC）的体外特性以及储存9周后的体内24小时活力。本研究的目的是确定谷氨酰胺酶作用于谷氨酰胺所产生的NH4+是否可以替代添加的NH4+盐。将浓缩红细胞与等体积的含有10 mM谷氨酰胺（EAS 31）、或10 mM谷氨酰胺加10 mM（EAS 36）或20 mM（EAS 37）Pi的腺嘌呤、葡萄糖、甘露醇和柠檬酸盐添加剂溶液一起储存。其中一份等分试样与Adsol一起储存。在28天时，储存在含谷氨酰胺加磷酸盐的EAS中的红细胞平均ATP水平分别为初始水平的132%（10 mM Pi）和144%（20 mM Pi），在84天时分别保持在48%和56%。储存在Adsol中的红细胞ATP水平在储存28天和84天时分别为105%和25%。与储存在Adsol中的红细胞相比，储存在谷氨酰胺以及谷氨酰胺加磷酸盐中的红细胞溶血和囊泡形成百分比显著更低（p < 0.01）。在储存84天时，EAS中的NH4+水平比Adsol中的高22%至34%，这表明谷氨酰胺被谷氨酰胺酶分解产生NH4+。在整个储存过程中，EAS 36和37中的红细胞平均体积（MCV）显著高于Adsol中的红细胞（p < 0.01）。（摘要截短于250字）
