Abrahamsson S O, Gelberman R
Massachusetts General Hospital, Department of Orthopedics, Boston 02114-2600.
Acta Orthop Scand. 1994 Oct;65(5):548-52. doi: 10.3109/17453679409000912.
To determine whether there are differences in the extent to which the superficial cellular layers remain viable and contribute to the healing process, extra- and intrasynovial hindlimb donor tendons of 12 dogs (24 tendons) were labeled with 3H-thymidine in vitro and transferred to the medial and lateral toes of the forepaw. After transplantation, the distribution and amounts of remaining labeled cells within the healing tendon grafts were determined at 1 and 3 weeks after surgery by scintillation countings and autoradiographic examinations. The two types of tendon grafts showed different turnover rates for the elimination of labeled DNA. At 1 week, 25 percent of labeled DNA remained in the extrasynovial grafts and 65 percent in the intrasynovial grafts. The two types of tendon grafts also showed different healing responses histologically. Autradiographic examinations showed that the majority of labeled cells were located in the periphery of the control tendons and that the numbers of labeled cells within the newly proliferated superficial areas of both types of tendon grafts decreased over time. These findings indicate that intrasynovial flexor tendons may possess improved prerequisites for superficial cellular survival and tendon gliding following autogenous tendon grafting.
为了确定滑膜浅层细胞存活并对愈合过程产生影响的程度是否存在差异，对12只犬（24条肌腱）的滑膜外和滑膜内后肢供体肌腱进行体外3H-胸腺嘧啶核苷标记，然后移植到前爪的内侧和外侧趾。移植后，通过闪烁计数和放射自显影检查，在术后1周和3周时测定愈合肌腱移植物中剩余标记细胞的分布和数量。两种类型的肌腱移植物在消除标记DNA方面显示出不同的更新率。术后1周时，滑膜外移植物中25%的标记DNA留存，滑膜内移植物中留存65%。两种类型的肌腱移植物在组织学上也表现出不同的愈合反应。放射自显影检查显示，大多数标记细胞位于对照肌腱的周边，且两种类型肌腱移植物新增殖浅层区域内的标记细胞数量均随时间减少。这些发现表明，自体肌腱移植后，滑膜内屈肌腱在浅层细胞存活和肌腱滑动方面可能具有更好的条件。
