Yang X Q, Shen S R, Hou J W, Zhao B L, Xin W J
Department of Tea Science, Zhejiang Agricultural University, Hangzhou, China.
Zhongguo Yao Li Xue Bao. 1994 Jul;15(4):350-3.
The concentration of 50% scavenging ratio (SC50) and the scavenging rate constants (k) and stiochiometric factor (n) were determined when (-)-epigallocatechin gallate [(-)-EGCG] scavenging superoxide anion free radical (O2.-) and hydroxyl radical (.OH). The mechanism of scavenging active free radicals of (-)-EGCG and the promotion of (-)-EGCG free radical and its structure were analyzed in vitro. Our results suggest that the centers of scavenging reaction of (-)-EGCG are B, D, and A cycles, and each (-)-EGCG traps six O2.- or .OH. It is in accord with the value of stiochiometric factor n = 6.
测定了（-）-表没食子儿茶素没食子酸酯[（-）-EGCG]清除超氧阴离子自由基（O2.-）和羟基自由基（.OH）时的50%清除率浓度（SC50）、清除速率常数（k）和化学计量系数（n）。在体外分析了（-）-EGCG清除活性自由基的机制以及（-）-EGCG自由基与其结构之间的关系。我们的结果表明，（-）-EGCG的清除反应中心是B、D和A环，每个（-）-EGCG捕获6个O2.-或.OH。这与化学计量系数n = 6的值一致。
