Lopategui J R, Gaffey M J, Chan J K, Frierson H F, Sun L H, Bellafiore F J, Chang K L, Weiss L M
Department of Pathology, City of Hope National Medical Center, Duarte, CA 91010.
Am J Surg Pathol. 1995 Jan;19(1):42-9. doi: 10.1097/00000478-199501000-00006.
CD30 (Ki-1)-positive anaplastic large cell lymphoma (CD30+ ALCL) is a morphologically and immunophenotypically distinct subset of non-Hodgkin's lymphoma. Although the presence of Epstein-Barr virus (EBV) has been well documented in a significant proportion of cases of Hodgkin's disease, another CD30+ malignancy, few studies have examined the association of EBV with CD30+ ALCL. These latter studies have produced conflicting findings. To further investigate the existence of a putative association of EBV with CD30+ ALCL, and whether this association, if present, shows geographic variation, we examined 34 formalin-fixed, paraffin-embedded specimens from cases of CD30+ ALCL from the United States and Hong Kong. Immunophenotypically, 15 cases were of B lineage, 15 cases were of T lineage, one case expressed both B- and T-cell markers, and three were of null lineage. A highly sensitive in situ hybridization method was performed with use of an antisense oligonucleotide probe to the EBV-encoded RNA (EBER-1). EBV-RNA was identified in 3 of 14 CD30+ ALCL specimens from Hong Kong patients and in 1 of 20 from the American patients. The EBER-1 signal was present in all or virtually all of the tumor cell nuclei in the three EBV-RNA-positive CD30+ ALCL Hong Kong cases, but was only focally present in the single EBV-positive American case. The latent membrane protein-1 (LMP1) of EBV was identified in only one of the four positive cases, a Hong Kong case. Our results suggest that in contrast to Hodgkin's disease, EBV has no significant association with CD30+ ALCL.
CD30（Ki-1）阳性间变性大细胞淋巴瘤（CD30+ ALCL）是一种在形态学和免疫表型上都有独特特征的非霍奇金淋巴瘤亚型。尽管在相当一部分霍奇金病（另一种CD30+恶性肿瘤）病例中，爱泼斯坦-巴尔病毒（EBV）的存在已有充分记录，但很少有研究探讨EBV与CD30+ ALCL之间的关联。这些后期研究得出了相互矛盾的结果。为了进一步研究EBV与CD30+ ALCL之间可能存在的关联，以及这种关联（如果存在的话）是否存在地域差异，我们检查了来自美国和香港的34例CD30+ ALCL病例的福尔马林固定、石蜡包埋标本。免疫表型分析显示，15例为B细胞系，15例为T细胞系，1例同时表达B细胞和T细胞标志物，3例为裸细胞系。我们使用针对EBV编码RNA（EBER-1）的反义寡核苷酸探针进行了一种高度灵敏的原位杂交方法。在14例来自香港患者的CD30+ ALCL标本中有3例检测到EBV-RNA，在20例来自美国患者的标本中有1例检测到。在3例EBV-RNA阳性的香港CD30+ ALCL病例中，EBER-1信号存在于所有或几乎所有肿瘤细胞核中，但在唯一一例EBV阳性的美国病例中仅局灶性存在。EBV的潜伏膜蛋白-1（LMP1）仅在4例阳性病例中的1例（1例香港病例）中检测到。我们的结果表明，与霍奇金病不同，EBV与CD30+ ALCL无显著关联。
