Boban N, McCallum J B, Schedewie H K, Boban M, Kampine J P, Bosnjak Z J
Department of Anesthesiology, Medical College of Wisconsin, Milwaukee 53226.
Anesth Analg. 1995 Jan;80(1):127-34. doi: 10.1097/00000539-199501000-00022.
Although the sympathetic ganglion is an important site of peripheral regulation, few studies have examined the effect of anesthetics on synaptic transmission. In the present study we compared the actions of desflurane with those of isoflurane on synaptic transmission and neurotransmitter release in the stellate ganglion. In the electrophysiologic group, 14 stellate ganglia were isolated from adult mongrel dogs after halothane anesthesia, desheathed, and superfused with Krebs' solution. Compound action potentials (CAP) were induced by supramaximal stimulation of the preganglionic T3-ramus at a low frequency of 0.4 Hz and were recorded from the postganglionic ventral ansa subclaviae. Each ganglion was exposed to two levels of anesthetics (equivalent to 1 and 2 minimum alveolar anesthetic concentration [MAC]), followed by an anesthetic-free washout period. While equianesthetic concentrations of isoflurane and desflurane caused essentially equipotent suppression of ganglionic transmission, desflurane was more efficacious than isoflurane, both with respect to the onset of and recovery from the inhibition of synaptic activity. In the electrochemical group, 25 ganglia were exposed to both anesthetics at a high concentration (equivalent to between 1.82 and 1.95 MAC) during maximal and submaximal current stimulations, and the release of actylcholine (ACh) in the superfusate was measured with liquid chromatography. Although desflurane and isoflurane caused a significant depression of CAP, neither anesthetic inhibited the release of ACh in the superfusate at either maximal or submaximal current stimulations. These results indicate that the suppression of ganglionic activity is equipotent for both anesthetics based on equivalent MAC values, but that desflurane is more efficacious than isoflurane with respect to onset and recovery at the higher concentrations of anesthetics.(ABSTRACT TRUNCATED AT 250 WORDS)
虽然交感神经节是外周调节的重要部位，但很少有研究考察麻醉剂对突触传递的影响。在本研究中，我们比较了地氟烷和异氟烷对星状神经节突触传递和神经递质释放的作用。在电生理组中，在氟烷麻醉后从成年杂种犬分离出14个星状神经节，去除被膜，并用 Krebs 溶液灌流。通过以0.4 Hz 的低频对节前 T3 支进行超强刺激诱发出复合动作电位（CAP），并从节后锁骨下襻前支记录。每个神经节暴露于两种麻醉剂水平（相当于1和2个最低肺泡麻醉浓度 [MAC]），随后是无麻醉剂的洗脱期。虽然等效麻醉浓度的异氟烷和地氟烷对神经节传递的抑制基本等效，但在地氟烷在抑制突触活动的起效和恢复方面比异氟烷更有效。在电化学组中，25个神经节在最大和次最大电流刺激期间暴露于高浓度的两种麻醉剂（相当于1.82至1.95 MAC 之间），并用液相色谱法测量灌流液中乙酰胆碱（ACh）的释放。虽然地氟烷和异氟烷导致 CAP 显著降低，但在最大或次最大电流刺激下，两种麻醉剂均未抑制灌流液中 ACh 的释放。这些结果表明，基于等效 MAC 值，两种麻醉剂对神经节活动的抑制是等效的，但在地氟烷在较高麻醉剂浓度下的起效和恢复方面比异氟烷更有效。（摘要截断于250字）
