Guitton J, Durand D, Bouillod N, Manchon M
Laboratoire des Urgences Biochimiques et Toxicologiques, Service de Biochimie (MP Baltassat), Hôpital Jules-Courmont, Centre Hospitalier Lyon Sud, Pierre Bénite, France.
Ann Biol Clin (Paris). 1994;52(2):111-5.
The aim of this work was to develop a procedure for analysis of drugs in serum using a chromatographic apparatus marketed by the Bio-Rad company. This automated apparatus, the Remedi, can identify nearly 200 substances and metabolites in biological fluids. The method for pretreatment of serum recommended by the manufacturer is inefficient. We thus decided to develop and validate an extraction method for analysis of serum. Our method extends column life and improves the sensitivity of the Remedi for the detection of drugs. For psychotropic drugs, a study was carried out that enables a semi-quantitative evaluation of blood levels to be made. In addition, the sera of patients admitted to hospital for intentional drug overdosage were studied to compare the results obtained using the Remedi and Emit techniques, and also to compare these results with toxicological data obtained by questioning the patient and his immediate circle.
这项工作的目的是开发一种使用伯乐公司销售的色谱仪器分析血清中药物的方法。这种自动化仪器Remedi能够识别生物体液中近200种物质和代谢物。制造商推荐的血清预处理方法效率低下。因此，我们决定开发并验证一种用于血清分析的提取方法。我们的方法延长了色谱柱寿命，并提高了Remedi检测药物的灵敏度。对于精神药物，进行了一项研究，能够对血药浓度进行半定量评估。此外，还对因故意药物过量入院的患者的血清进行了研究，以比较使用Remedi和Emit技术获得的结果，并将这些结果与通过询问患者及其直系亲属获得的毒理学数据进行比较。
