Takada Y, Shinkai F, Kondo S, Yamamoto S, Tsuboi H, Korenaga R, Ando J
Institute for Life Science Research, Asahi Chemical Industry Co., Ltd., Fuji-City, Japan.
Biochem Biophys Res Commun. 1994 Dec 15;205(2):1345-52. doi: 10.1006/bbrc.1994.2813.
Endothelial cells (ECs) cultured from human umbilical vein were exposed to medium flow in a flow-loading chamber, and changes in thrombomodulin (TM) expression were examined by flow cytometry and enzyme linked immunosorbent assay with monoclonal antibody. The expression of TM antigen was increased time- and shear stress-dependently by flow, and when exposed to a shear stress of 15 dynes/cm2 for 24 hr, it increased to approximately 200% of the stationary control level. Reverse transcriptase-polymerase chain reaction showed that TM mRNA levels in ECs also increased in response to flow. TM mRNA began to increase one hour after the application of shear stress of 15 dynes/cm2 and reached a maximum (approximately 330% of stationary control) after eight hours. These results, demonstrating an up-regulating effect of flow on TM expression in ECs, suggest that shear stress may be an important modulator of intravascular blood coagulation.
将人脐静脉培养的内皮细胞（ECs）置于流动加载室中使其暴露于介质流中，并用单克隆抗体通过流式细胞术和酶联免疫吸附测定法检测血栓调节蛋白（TM）表达的变化。TM抗原的表达随时间和剪切应力而呈依赖性增加，当暴露于15达因/平方厘米的剪切应力24小时时，其增加至静态对照水平的约200%。逆转录聚合酶链反应表明，ECs中的TM mRNA水平也随流动而增加。在施加15达因/平方厘米的剪切应力1小时后，TM mRNA开始增加，并在8小时后达到最大值（约为静态对照的330%）。这些结果表明流动对ECs中TM表达具有上调作用，提示剪切应力可能是血管内血液凝固的重要调节因子。
