用反向被动血凝反应鉴定A群链球菌

Identification of group a streptococci by reverse passive hemagglutination.

作者信息

Edwards E A, Larson G L

出版信息

Immunol Commun. 1976;5(1-2):41-51. doi: 10.3109/08820137609020611.

DOI:10.3109/08820137609020611

Abstract

A method for preparing sensitized human O erythrocytes with specific antibody is reported. Whole sera or 40% ammonium sulfate insoluble globulin fractions did not satisfactorily sensitize erythrocytes to agglutinate in the presence of either group specific polysaccharide of group A beta-hemolytic strepticocci or antigen produced during colony formation. Antibody purified by affinity chromatography sensitized the erythrocytes to rapidly agglutinate in the presence of either antigen. No spontaneous or "pseudo-immune" agglutination occured when the sensitized erythrocytes were suspended in human serum. Such sensitized human cells, while more difficult to prepare than sensitized staphylococci, should be suitable for detecting bacterial or viral antigens in vivo.

摘要

报道了一种用特异性抗体制备致敏人O型红细胞的方法。在A群β溶血性链球菌的群特异性多糖或菌落形成过程中产生的抗原存在的情况下，全血清或40%硫酸铵不溶性球蛋白组分不能令人满意地使红细胞致敏以发生凝集。通过亲和层析纯化的抗体可使红细胞在任何一种抗原存在的情况下迅速凝集。当致敏红细胞悬浮于人体血清中时，未发生自发或“假免疫”凝集。这种致敏人细胞虽然比致敏葡萄球菌更难制备，但应适合于在体内检测细菌或病毒抗原。