SCHMIDT W C, MOORE D J
J Exp Med. 1965 May 1;121(5):793-806. doi: 10.1084/jem.121.5.793.
A hemagglutination method employing tanned human O erythrocytes sensitized with purified Group A streptococcal polysaccharide has been developed to measure A polysaccharide antibody in antistreptococcal rabbit and human sera. The reaction of sensitized RBC with known Group A streptococcal rabbit antisera, inhibition of hemagglutination with A polysaccharide, and abolition of hemagglutination and hemagglutination-inhibition by exposure of polysaccharide antigen to A enzyme, which destroys the specific immunologic determinant of A carbohydrate, were utilized to demonstrate the specificity of this hemagglutinating system. The results of A polysaccharide antibody determinations on sera from normal persons, patients convalescent from streptococcal infection, and patients with non-suppurative streptococcal sequelae are presented.
已开发出一种血凝方法,该方法采用经纯化的A组链球菌多糖致敏的鞣酸处理人O型红细胞,以测定抗链球菌兔血清和人血清中的A多糖抗体。利用致敏红细胞与已知A组链球菌兔抗血清的反应、A多糖对血凝的抑制作用,以及通过将多糖抗原暴露于A酶(该酶可破坏A碳水化合物的特异性免疫决定簇)来消除血凝和血凝抑制,来证明该血凝系统的特异性。文中给出了对正常人、链球菌感染康复患者以及非化脓性链球菌后遗症患者血清中A多糖抗体的测定结果。