Garicochea B, van Rhee F, Spencer A, Chase A, Lin F, Cross N C, Goldman J M
LRF Centre for Adult Leukaemia, London.
Br J Haematol. 1994 Oct;88(2):400-2. doi: 10.1111/j.1365-2141.1994.tb05039.x.
We report a patient with CML in relapse after a sex-mismatch bone marrow transplant who was treated with donor lymphocyte infusions and developed severe marrow aplasia 3 months later. As cytogenetic analysis at this point was not possible because of the very low number of marrow cells available, we used in situ hybridization (ISH) for sex chromosomes and the BCR/ABL gene together with quantitative PCR to monitor the patient's response. The results suggest that recovering haemopoiesis was derived from donor cells and contributed to the decision not to transfuse donor marrow cells on a second occasion.
我们报告了一名慢性粒细胞白血病（CML）患者，其在性别不匹配的骨髓移植后复发，接受了供体淋巴细胞输注治疗，3个月后出现严重骨髓再生障碍。由于当时可获得的骨髓细胞数量极少，无法进行细胞遗传学分析，我们使用性染色体原位杂交（ISH）和BCR/ABL基因检测以及定量PCR来监测患者的反应。结果表明，造血功能的恢复源自供体细胞，这一结果促使我们决定不再二次输注供体骨髓细胞。
