影响大肠杆菌中镁运输的三个基因的突变体：遗传学与生理学
Mutants in three genes affecting transport of magnesium in Escherichia coli: genetics and physiology.
作者信息
Park M H, Wong B B, Lusk J E
出版信息
J Bacteriol. 1976 Jun;126(3):1096-103. doi: 10.1128/jb.126.3.1096-1103.1976.
Abstract
Mutants in three genes affecting two Mg2+ transport systems are described. System I, for which Co2+, Mn2+, and Mg2+ are substrates, is inactive in corA mutants corB mutants express system I after growth on high (10 mM) Mg2+ but not low (0.1 mM) Mg2+. Both corA and corB mutants are resistant to Co2+ or Mn2+. corA mutants are sensitive to CA2+. Transport system II is specific for Mg2+ and is repressed by growth on 10 mM Mg2+. mgt mutations inactivate system II. Growth on mgt mutants in normal except on very low (1 muM) concentrations of Mg2+, corA mgt strains exhibit no high-affinity, energy-dependent transport of Mg2+ and require 10 mM Mg2+ for optimal growth. The three genes are not linked. The corA locus is contransducible with ilv at 75 min, corB is cotransducible with pyrB at 85 min, and mgt is cotransducible with malB and mel at 81 min on the genetic map.
摘要
本文描述了影响两种镁离子转运系统的三个基因的突变体。系统I以钴离子、锰离子和镁离子作为底物，在corA突变体中无活性；corB突变体在高镁（10 mM）环境中生长后可表达系统I，但在低镁（0.1 mM）环境中则不能。corA和corB突变体均对钴离子或锰离子具有抗性。corA突变体对钙离子敏感。转运系统II对镁离子具有特异性，并在10 mM镁离子环境中生长时受到抑制。mgt突变会使系统II失活。mgt突变体在正常条件下生长，除非镁离子浓度极低（1 μM），corA mgt菌株不具备高亲和力、能量依赖性的镁离子转运能力，且需要10 mM镁离子才能实现最佳生长。这三个基因不连锁。在遗传图谱上，corA位点与ilv在75分钟时共转导，corB与pyrB在85分钟时共转导，mgt与malB和mel在81分钟时共转导。
相似文献
1
Mutants in three genes affecting transport of magnesium in Escherichia coli: genetics and physiology.影响大肠杆菌中镁运输的三个基因的突变体：遗传学与生理学
J Bacteriol. 1976 Jun;126(3):1096-103. doi: 10.1128/jb.126.3.1096-1103.1976.
2
Magnesium transport in Salmonella typhimurium: genetic characterization and cloning of three magnesium transport loci.鼠伤寒沙门氏菌中的镁转运：三个镁转运基因座的遗传特征分析与克隆
J Bacteriol. 1989 Sep;171(9):4742-51. doi: 10.1128/jb.171.9.4742-4751.1989.
3
Magnesium transport in Salmonella typhimurium: characterization of magnesium influx and cloning of a transport gene.鼠伤寒沙门氏菌中的镁转运：镁流入的特征及一个转运基因的克隆
J Bacteriol. 1986 Dec;168(3):1444-50. doi: 10.1128/jb.168.3.1444-1450.1986.
4
Magnesium transport in Salmonella typhimurium: the influence of new mutations conferring Co2+ resistance on the CorA Mg2+ transport system.鼠伤寒沙门氏菌中的镁转运：赋予Co2+抗性的新突变对CorA Mg2+转运系统的影响。
Mol Microbiol. 1991 Nov;5(11):2753-62. doi: 10.1111/j.1365-2958.1991.tb01984.x.
5
Manganese-resistant mutants of Escherichia coli: physiological and genetic studies.大肠杆菌的耐锰突变体：生理学和遗传学研究
J Bacteriol. 1972 Apr;110(1):186-95. doi: 10.1128/jb.110.1.186-195.1972.
6
Magnesium transport in Salmonella typhimurium: 28Mg2+ transport by the CorA, MgtA, and MgtB systems.鼠伤寒沙门氏菌中的镁转运：通过CorA、MgtA和MgtB系统进行的28Mg2+转运。
J Bacteriol. 1989 Sep;171(9):4761-6. doi: 10.1128/jb.171.9.4761-4766.1989.
7
Magnesium uptake of Arabidopsis transporters, AtMRS2-10 and AtMRS2-11, expressed in Escherichia coli mutants: Complementation and growth inhibition by aluminum.在大肠杆菌突变体中表达的拟南芥转运蛋白AtMRS2 - 10和AtMRS2 - 11对镁的摄取：铝的互补作用和生长抑制
Biochim Biophys Acta. 2015 Jun;1848(6):1376-82. doi: 10.1016/j.bbamem.2015.03.005. Epub 2015 Mar 13.
8
Properties of Escherichia coli mutants altered in calcium/proton antiport activity.钙/质子反向转运活性发生改变的大肠杆菌突变体的特性
J Bacteriol. 1979 Sep;139(3):824-34. doi: 10.1128/jb.139.3.824-834.1979.
9
10
The homologous Arabidopsis MRS2/MGT/CorA-type Mg channels, AtMRS2-10 and AtMRS2-1 exhibit different aluminum transport activity.同源拟南芥 MRS2/MGT/CorA 型镁通道 AtMRS2-10 和 AtMRS2-1 表现出不同的铝转运活性。
Biochim Biophys Acta Biomembr. 2018 Nov;1860(11):2184-2191. doi: 10.1016/j.bbamem.2018.08.016. Epub 2018 Aug 31.
引用本文的文献
1
High-Throughput Tn-Seq Screens Identify Both Known and Novel Pseudomonas putida KT2440 Genes Involved in Metal Tolerance.高通量转座子测序筛选鉴定出恶臭假单胞菌KT2440中已知和新的与金属耐受性相关的基因。
Environ Microbiol. 2025 May;27(5):e70095. doi: 10.1111/1462-2920.70095.
2
Regulation of magnesium ion transport in : insights into the role of the 5' upstream region in expression.调控镁离子转运体：5'上游区在基因表达中的作用研究。
RNA Biol. 2024 Jan;21(1):94-106. doi: 10.1080/15476286.2024.2421665. Epub 2024 Nov 8.
3
The role of metals in hypothiocyanite resistance in .金属在. 对次碘酸盐抗性中的作用
J Bacteriol. 2024 Aug 22;206(8):e0009824. doi: 10.1128/jb.00098-24. Epub 2024 Jul 17.
4
The role of metals in hypothiocyanite resistance in .金属在……中对次硫氰酸盐抗性的作用。 （原文句子不完整，翻译可能不太准确，你可补充完整原文以便更精准翻译）
bioRxiv. 2024 Mar 8:2024.03.07.583962. doi: 10.1101/2024.03.07.583962.
5
Magnesium Hydroxide Nanoparticles Kill Exponentially Growing and Persister Cells by Causing Physical Damage.氢氧化镁纳米颗粒通过造成物理损伤杀死指数生长期和持留菌细胞。
Nanomaterials (Basel). 2021 Jun 16;11(6):1584. doi: 10.3390/nano11061584.
6
TRPM channels and magnesium in early embryonic development.瞬时受体电位M型通道与早期胚胎发育中的镁
Int J Dev Biol. 2015;59(7-9):281-8. doi: 10.1387/ijdb.150196lr.
7
Solute Carrier Family SLC41, what do we really know about it?溶质载体家族SLC41，我们对它究竟了解多少？
Wiley Interdiscip Rev Membr Transp Signal. 2013;2(6). doi: 10.1002/wmts.95.
8
The unique nature of mg2+ channels.镁离子通道的独特性质。
Physiology (Bethesda). 2008 Oct;23:275-85. doi: 10.1152/physiol.00019.2008.
9
Ni2+-uptake in Pseudomonas putida strain S4: a possible role of Mg2+-uptake pump.恶臭假单胞菌S4菌株对镍离子的摄取：镁离子摄取泵的潜在作用
J Biosci. 2006 Mar;31(1):61-7. doi: 10.1007/BF02705236.
10
CorA affects tolerance of Escherichia coli and Salmonella enterica serovar Typhimurium to the lactoperoxidase enzyme system but not to other forms of oxidative stress.CorA影响大肠杆菌和鼠伤寒沙门氏菌对乳过氧化物酶系统的耐受性，但不影响它们对其他形式氧化应激的耐受性。
Appl Environ Microbiol. 2005 Nov;71(11):6515-23. doi: 10.1128/AEM.71.11.6515-6523.2005.
本文引用的文献
1
Manganese Active Transport in Escherichia coli.大肠杆菌中的锰主动运输
J Bacteriol. 1970 Dec;104(3):1299-306. doi: 10.1128/jb.104.3.1299-1306.1970.
2
CATION TRANSPORT IN ESCHERICHIA COLI. IV. KINETICS OF NET K UPTAKE.大肠杆菌中的阳离子转运。IV. 净钾摄取动力学
J Gen Physiol. 1963 Nov;47(2):329-46. doi: 10.1085/jgp.47.2.329.
3
Magnesium transport in Escherichia coli.大肠杆菌中的镁转运
J Biol Chem. 1971 Feb 10;246(3):569-76.
4
Cation transport in Escherichia coli. VI. K exchange.大肠杆菌中的阳离子转运。VI. 钾离子交换
J Gen Physiol. 1966 Jan;49(3):469-81. doi: 10.1085/jgp.49.3.469.
5
Magnesium and the growth of Escherichia coli.镁与大肠杆菌的生长
J Biol Chem. 1968 May 25;243(10):2618-24.
6
Magnesium transport in Escherichia coli. Inhibition by cobaltous ion.大肠杆菌中的镁转运。钴离子的抑制作用。
J Biol Chem. 1971 May 10;246(9):3042-9.
7
Manganese accumulation by Escherichia coli: evidence for a specific transport system.大肠杆菌对锰的积累：特定转运系统的证据。
Biochem Biophys Res Commun. 1969 Mar 10;34(5):640-5. doi: 10.1016/0006-291x(69)90786-4.
8
Magneisum transport in Escherichia coli.大肠杆菌中的镁转运
J Biol Chem. 1969 Mar 25;244(6):1653-5.
9
Escherichia coli K-12 F-prime factors, old and new.大肠杆菌K-12 F-prime因子，新旧情况
Bacteriol Rev. 1972 Dec;36(4):587-607. doi: 10.1128/br.36.4.587-607.1972.
10
Linkage map of Escherichia coli strain K-12.大肠杆菌K-12菌株的连锁图谱。
Bacteriol Rev. 1972 Dec;36(4):504-24. doi: 10.1128/br.36.4.504-524.1972.
Zotero 插件