Epstein W, Schultz S G
J Gen Physiol. 1966 Jan;49(3):469-81. doi: 10.1085/jgp.49.3.469.
K influx and net K flux have been measured in suspensions of chloramphenicol-arrested Escherichia coli. The rate of K exchange in the steady state was independent of the K concentration of the medium over a 200-fold range. Under a number of experimental conditions the rate of exchange may be considerably increased or decreased without changing the cellular K content. These results show that under these conditions changes in K influx are associated with equal changes in K efflux, and suggest that the latter process is, at least in part, both carrier-mediated and tightly coupled to the influx process.
已在氯霉素抑制的大肠杆菌悬液中测量了钾离子流入量和净钾离子通量。在200倍的浓度范围内,稳态下钾离子交换速率与培养基中的钾离子浓度无关。在许多实验条件下,交换速率可能会大幅增加或降低,而细胞内钾含量不变。这些结果表明,在这些条件下,钾离子流入量的变化与钾离子流出量的等量变化相关,这表明后者至少部分是由载体介导的,并且与流入过程紧密耦合。