Ye B, Li Q, Li Y, Yu J
Research Institute of Biochemical Engineering, East China University of Science and Technology, Shanghai.
Chin J Biotechnol. 1994;10(2):83-9.
A micro-enzyme electrode was fabricated by cross-linking L-glutamate oxidase with glutaraldehyde on aminopropyl-platinized platinum wire. A flow injection analysis system with glutamate sensor was used for L-glutamate determination. The peak current is linearly related to the L-glutamate concentration in the range of 0.02-2.0 mM, with good performance, accuracy (CV = 0.4%), fast response (< 60s), and stability (> 20 days). The system was applied to determine the concentration of L-glutamate in a fermentation broth. The recovery rate was in the range of 98.7-107.5%.
通过在氨丙基镀铂铂丝上用戊二醛交联L-谷氨酸氧化酶制备了一种微酶电极。使用带有谷氨酸传感器的流动注射分析系统测定L-谷氨酸。在0.02 - 2.0 mM范围内，峰电流与L-谷氨酸浓度呈线性关系，具有良好的性能、准确性（CV = 0.4%）、快速响应（< 60秒）和稳定性（> 20天）。该系统用于测定发酵液中L-谷氨酸的浓度。回收率在98.7 - 107.5%范围内。
