Coucke P H, De Pauw-Gillet M C, Bonjean K A, Siwek B L, Foidart J M, Bassleer R J
Laboratory of Histology, University of Liège, Belgium.
In Vivo. 1994 May-Jun;8(3):333-8.
A parental line of mouse B16 melanoma cells (B16) and two derived cloned lines, either pigmented (B16P) or non pigmented (B16NP), were cultured in vitro as spheroids. After 48 hrs, the pigmented cells (B16, B16P) formed smaller and looser aggregates, with higher rates of cell proliferation and lower amounts of extracellular matrix as compared to B16NP spheroids. The three lines were more tumorigenic when inoculated subcutaneously as spheroids than as isolated cells. Furthermore, B16P or B16 spheroids developed richly vascularized subcutaneous tumors and metastases more rapidly than B16NP aggregates. After intravenous injection of spheroids, the measurement with an image analyzer of the area of sections in lung colonies indicated that B16P colonies were larger and more numerous than those induced by B16NP cells.
小鼠B16黑色素瘤细胞的亲代细胞系（B16）以及两个衍生的克隆细胞系，即色素沉着的（B16P）或无色素的（B16NP），作为球体在体外培养。48小时后，与B16NP球体相比，色素沉着细胞（B16、B16P）形成的聚集体更小、更松散，细胞增殖率更高，细胞外基质含量更低。当作为球体皮下接种时，这三个细胞系比作为分离细胞时更具致瘤性。此外，B16P或B16球体比B16NP聚集体更快地形成血管丰富的皮下肿瘤和转移灶。静脉注射球体后，用图像分析仪测量肺集落切片的面积表明，B16P集落比B16NP细胞诱导的集落更大、更多。
