体腔液DNA流式细胞术分析与传统细胞学的比较。

Comparison of DNA flow cytometric analysis of body cavity fluids with conventional cytology.

作者信息

Lima C E, Mizushima Y, Masuda S, Kitagawa M

机构信息

First Department of Pathology, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan.

出版信息

In Vivo. 1994 May-Jun;8(3):359-62.

PMID:7803718

Abstract

The potential utility of flow cytometric (FCM) analysis in routine diagnostic cytology for effusions was assessed with 104 samples from body cavity fluids. Conventional cytology showed no positivity (0/33) for effusions from patients with non-malignant diseases, and 45% positivity (38/71) for effusions from patients with malignant diseases. Conversely, FCM showed no positivity (aneuploid) (0/33) for effusions from patients with non-malignant diseases, and 18% positivity (13/71) with malignant diseases. In 13 cases examined, 8 cases (62%) showed an identical DNA ploidy pattern between effusion site and primary site. FCM showed positivity in four cytologically negative samples (3 malignant lymphomas and 1 clear cell carcinoma). In cases with malignant lymphoma, conventional cytology showed 40% positivity (4/10) and FCM 60% (6/10) positivity. These results indicate that DNA analysis of cells in malignant effusions does not have a diagnostic value as a routine test, but it may have in cases of malignant lymphoma.

摘要

利用104份体腔积液样本评估了流式细胞术（FCM）分析在常规诊断性积液细胞学中的潜在效用。传统细胞学检查显示，非恶性疾病患者的积液无阳性结果（0/33），恶性疾病患者的积液阳性率为45%（38/71）。相反，FCM显示，非恶性疾病患者的积液无阳性结果（非整倍体）（0/33），恶性疾病患者的积液阳性率为18%（13/71）。在检查的13例病例中，8例（62%）积液部位和原发部位之间的DNA倍体模式相同。FCM在4例细胞学检查阴性的样本中呈阳性（3例恶性淋巴瘤和1例透明细胞癌）。在恶性淋巴瘤病例中，传统细胞学检查的阳性率为40%（4/10），FCM的阳性率为60%（6/10）。这些结果表明，恶性积液细胞的DNA分析作为一项常规检查没有诊断价值，但在恶性淋巴瘤病例中可能有诊断价值。