基于单克隆抗体的竞争性酶联免疫吸附测定法，用于检测不同动物物种血清中针对伯氏考克斯氏体的特异性抗体。

Monoclonal antibody based competitive ELISA for the detection of specific antibodies against Coxiella burnetii in sera from different animal species.

作者信息

Jaspers U, Thiele D, Krauss H

机构信息

Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Giessen, Germany.

出版信息

Zentralbl Bakteriol. 1994 Jun;281(1):61-6. doi: 10.1016/s0934-8840(11)80638-9.

DOI:10.1016/s0934-8840(11)80638-9

PMID:7803931

Abstract

A competitive ELISA system for the detection of antibodies against Coxiella (C.) burnetii in cattle, sheep, goats, horses and humans is described. The ELISA is based on a biotinylated monoclonal antibody with specificity for C. burnetii lipopolysaccharide in combination with streptavidin peroxidase. For evaluation and statistical analysis, 413 sera from cattle, sheep, goats, horses and humans were tested in parallel in the indirect immunofluorescence test (IFT). Furthermore, a total of 448 bovine and human sera were also tested with an indirect ELISA and 47 sheep sera were investigated using the commercially available "Ridascreen AK EIA". Sensitivity and specificity values for the competitive ELISA described and determined with the aid of the indirect immunofluorescence test (IFT) as reference were: cattle, 88% and 89%; sheep, 100% and 93%, goat, 82% and 96%, horse 100% and 93%, and man 42% and 96%, respectively.

摘要

本文描述了一种用于检测牛、羊、山羊、马和人类中抗伯氏考克斯体（C. burnetii）抗体的竞争性酶联免疫吸附测定（ELISA）系统。该ELISA基于对伯氏考克斯体脂多糖具有特异性的生物素化单克隆抗体，并结合链霉亲和素过氧化物酶。为了进行评估和统计分析，对来自牛、羊、山羊、马和人类的413份血清同时进行了间接免疫荧光试验（IFT）检测。此外，还使用间接ELISA对448份牛和人类血清进行了检测，并使用市售的“Ridascreen AK EIA”对47份绵羊血清进行了研究。以间接免疫荧光试验（IFT）为参照，所描述的竞争性ELISA的灵敏度和特异性值分别为：牛，88%和89%；绵羊，100%和93%；山羊，82%和96%；马，100%和93%；人类，42%和96%。