Nunes L R, Teixeira M M, Camargo E P, Affonso H T
Departamento de Parasitologia, Universidade de São Paulo, Brasil.
J Eukaryot Microbiol. 1994 Sep-Oct;41(5):496-500. doi: 10.1111/j.1550-7408.1994.tb06048.x.
Hybridization using kDNA and rDNA sequences as probes was performed to study phylogenetic relatedness of different species of trypanosomatids. Using this approach, we identified five organisms which had been classified as Phytomonas and Herpetomonas that were more closely correlated to each other phylogenetically than to any other species or isolates from either genera. These findings raise doubts about the validity of the current classification of Trypanosomatidae. Finally, we demonstrated the usefulness of kDNA sequences as an alternative to genomic sequences in obtaining phylogenetic information on trypanosomatids.
使用 kDNA 和 rDNA 序列作为探针进行杂交，以研究不同锥虫物种之间的系统发育相关性。通过这种方法，我们鉴定出了五种被归类为植滴虫属和曲滴虫属的生物体，它们在系统发育上彼此之间的相关性比与这两个属的任何其他物种或分离株都更为密切。这些发现对当前锥虫科分类的有效性提出了质疑。最后，我们证明了 kDNA 序列在获取锥虫系统发育信息方面可作为基因组序列的替代方法。
