Teixeira M M, Takata C S, Conchon I, Campaner M, Camargo E P
Departamento de Parasitologia, Instituto de Ciencias Biomédicas, Universidade de São Paulo, Brazil.
J Parasitol. 1997 Feb;83(1):58-65.
We examined files of various families for the presence of trypanosomatids. Of 592 insects, 113 (19%) were positive. From these insects, we obtained 42 cultures and selected 14 for further analysis. Seven of the cultures had the characteristics of Herpetomonas species, they displayed typical opisthomastigotes, lacked arginase, possessed a Pvu II restriction site at 360 bp from the 5' end of the small subunit ribosomal gene, and did not possess a Hin dIII site at 1,500 bp from the ribosomal alpha-large subunit 5' end. Hybridization with synthetic oligonucleotides complementary to the sequences flanking the Pvu II site in Herpetomonas samuelpessoai and Herpetomonas muscarum, permitted the distribution of the old species and the 7 isolates into 2 subgroups of Herpetomonas spp. Of the remaining 7 cultures, 4 were probably Leptomonas spp., whereas the other 3, together with Herpetomonas roitmani, seem to constitute a novel group with morphological and molecular characteristics quite distinct from those of Herpetomonas spp. or any other genera of Trypanosomatidae. We also studied some trypanosomatid species of questionable taxonomic status, Leptomonas samueli and Phytomonas davidi yielded results identical to those of Herpetomonas spp., thus confirming their already suspected affiliation to this genus. On the other hand, Herpetomonas anglusteri, Herpetomonas dedonderi, and Herpetomonas mcgheei displayed morphological and molecular characteristics incompatible with their placement in the genus Herpetomonas.
我们检查了不同家族的档案，以确定锥虫的存在。在592只昆虫中，113只（19%）呈阳性。从这些昆虫中，我们获得了42个培养物，并选择了14个进行进一步分析。其中7个培养物具有赫氏鞭毛虫属物种的特征，它们显示出典型的后鞭毛体，缺乏精氨酸酶，在小亚基核糖体基因5'端360 bp处有一个Pvu II限制性位点，并且在核糖体α大亚基5'端1500 bp处没有Hin dIII位点。与与赫氏鞭毛虫属的赫氏塞缪尔鞭毛虫和赫氏家蝇的Pvu II位点侧翼序列互补的合成寡核苷酸杂交，使得旧物种和7个分离株能够被分为赫氏鞭毛虫属的2个亚组。在其余的7个培养物中，4个可能是细滴虫属物种，而另外3个与鲁伊特曼氏赫氏鞭毛虫一起，似乎构成了一个新的类群，其形态和分子特征与赫氏鞭毛虫属或锥虫科的任何其他属都有很大不同。我们还研究了一些分类地位存疑的锥虫物种，塞缪尔氏细滴虫和大卫氏植滴虫的结果与赫氏鞭毛虫属物种相同，从而证实了它们与该属已被怀疑的亲缘关系。另一方面，狭尾赫氏鞭毛虫、德东代氏赫氏鞭毛虫和麦吉氏赫氏鞭毛虫的形态和分子特征与它们被归为赫氏鞭毛虫属不相符。
