电离辐射诱导人肺癌细胞产生白细胞介素-1α、白细胞介素-6和粒细胞巨噬细胞集落刺激因子。
Ionizing radiation-induced IL-1 alpha, IL-6 and GM-CSF production by human lung cancer cells.
作者信息
Zhang J S, Nakatsugawa S, Niwa O, Ju G Z, Liu S Z
机构信息
MPH Radiobiology Research Unit, Norman Bethune University of Medical Sciences, Changchun.
出版信息
Chin Med J (Engl). 1994 Sep;107(9):653-7.
A cell line derived from human lung cancer (AOI) was employed in the present study. A panel of cytokines were quantified by ELISA technique following cellular exposure to X-irradiation. Tremendous increase in the levels of both IL-1 alpha and IL-6 were observed, GM-CSF was also detected. A comparison of time kinetics of IL-1 alpha and IL-6 production was made with that of cell cycle progression which was determined by FCM BrdU/DNA bivariate analysis. No cell cycle specific changes were found. The biological implication of radiation-induced cytokine production was discussed.
本研究采用了一种源自人肺癌的细胞系（AOI）。在细胞暴露于X射线照射后，通过酶联免疫吸附测定（ELISA）技术对一组细胞因子进行了定量分析。观察到白细胞介素-1α（IL-1α）和白细胞介素-6（IL-6）水平均大幅升高，同时也检测到了粒细胞-巨噬细胞集落刺激因子（GM-CSF）。通过流式细胞术（FCM）溴脱氧尿苷（BrdU）/DNA双变量分析确定细胞周期进程，并将IL-1α和IL-6产生的时间动力学与之进行了比较。未发现细胞周期特异性变化。文中讨论了辐射诱导细胞因子产生的生物学意义。
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