Cai J H, Jiang Y K, Zhang Y Y, Lu G H, Zhang X H, Gao Q Z, Zuo L F
Department of Surgery, Second Affiliated Hospital, Hebei Medical College, Shijiazhuang.
Chin Med J (Engl). 1994 Sep;107(9):683-7.
Quantitative analysis of ras oncogene product P21 was performed on paraffin blocks from 55 smooth muscle tumors of the gastrointestinal tract by immunofluorescence and flow cytometry. No positive evidence for P21 was found in 5 cases of normal smooth muscle tissues. Eight of 14 leiomyomas were P21 positive (57%) but all of them were lower expressed with fluorescence index (FI) < 1.25, whereas a total of 29 leiomyosarcomas and 12 potential malignant smooth muscle tumors were found to be P21 positive (100%), and the majority were overexpressed with FI > 1.25 (P < 0.005). ras P21 was overexpressed significantly in the tumors with mitotic counts of more than 1/10 high power field (P < 0.005). It was also overexpressed significantly as soon as the mild cytologic atypia of the tumor was found (P < 0.005). Five-year survival rate was significantly higher in the patients with lower-expression of ras P21 than those with overexpression (P < 0.005). It was suggested that the expression of ras oncogene product P21 could be used as an objective parameter in distinguishing malignants from benigns and predicting the prognosis of the patients with smooth muscle tumors of the gastrointestinal tract.
通过免疫荧光和流式细胞术对55例胃肠道平滑肌肿瘤石蜡块进行ras癌基因产物P21的定量分析。5例正常平滑肌组织中未发现P21阳性证据。14例平滑肌瘤中有8例P21阳性（57%），但荧光指数（FI）均<1.25，表达较低；而29例平滑肌肉瘤和12例潜在恶性平滑肌肿瘤均为P21阳性（100%），多数FI>1.25，呈过表达（P<0.005）。有丝分裂计数超过1/10高倍视野的肿瘤中，ras P21明显过表达（P<0.005）。肿瘤出现轻度细胞学异型性时，ras P21也明显过表达（P<0.005）。ras P21低表达患者的5年生存率明显高于过表达患者（P<0.005）。提示ras癌基因产物P21的表达可作为鉴别胃肠道平滑肌肿瘤良恶性及预测患者预后的客观指标。
